Recombinant Human UFM1 Rhodamine Protein, CF

Catalog # Availability Size / Price Qty
UL-522-050
Product Details
Citations (1)
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Recombinant Human UFM1 Rhodamine Protein, CF Summary

Purity
>95%, by PAGE
Activity
Recombinant Human Ufm1 Rhodamine is ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 570 nM and an emission wavelength of 590 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ufm1 Rhodamine concentration of 0.1-1 μM.
Source
E. coli-derived human UFM1 protein
Accession #

Product Datasheets

UL-522

Formulation X mg/ml (XµM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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Background: UFM1

Human Ubiquitin-fold Modifier 1 (UFM1), also known as BM-002, is an 85 amino acid (aa) member of the Ubiquitin-like protein family that has a predicted molecular weight of 9.1 kDa. Human and mouse UFM1 share 100% aa sequence identity and are primarily localized in the nucleus, but can also be detected in the cytoplasm and the endoplasmic reticulum (ER) (1). ER localization of UFM1 appears to be dependent on the co-expression of UFBP1 (2). Initially expressed as an inactive precursor, UFM1 undergoes proteolytic cleavage at the C-terminus to expose a conserved glycine residue that is necessary for UFM1 conjugation to target proteins. Conjugation of UFM1 to target proteins requires a UFM1-activating (E1) enzyme, a UFM1-conjugating (E2) enzyme, and a UFM1 ligase (E3) (1,3). UFM1 has been shown to be a mediator of ER stress-induced apoptosis and to promote the pathogenesis of Leishmania (2,4). This protein is modified with rhodamine red via primary amine coupling, resulting in the modification of lysine residues as well as the N-terminus. This labeled Ufm1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6-tag.

References
  1. Komatsu, M. et al. (2004) EMBO J. 23:1977.
  2. Lemaire, K. et al. (2011) PLoS One 6:e18517.
  3. Tatsumi, K. et al. (2010) J. Biol. Chem. 285:5417.
  4. Gannavaram, S. et al. (2012) Mol. Microbiol. 86:187.
 
Long Name
Ubiquitin-fold Modifier 1
Entrez Gene IDs
51569 (Human); 67890 (Mouse); 365797 (Rat)
Alternate Names
bA131P10.1; BM-002; C13orf20; chromosome 13 open reading frame 20; ubiquitin-fold modifier 1; UFM1

Citation for Recombinant Human UFM1 Rhodamine Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Characterization of the deubiquitination activity and substrate specificity of the chicken ubiquitin-specific protease 1/USP associated factor 1 complex
    Authors: H Zheng, M Wang, C Zhao, S Wu, P Yu, Y Lü, T Wang, Y Ai
    PLoS ONE, 2017;12(11):e0186535.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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