Recombinant Human UFM1 Rhodamine Protein, CF
Recombinant Human UFM1 Rhodamine Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (XµM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.|
Human Ubiquitin-fold Modifier 1 (UFM1), also known as BM-002, is an 85 amino acid (aa) member of the Ubiquitin-like protein family that has a predicted molecular weight of 9.1 kDa. Human and mouse UFM1 share 100% aa sequence identity and are primarily localized in the nucleus, but can also be detected in the cytoplasm and the endoplasmic reticulum (ER) (1). ER localization of UFM1 appears to be dependent on the co-expression of UFBP1 (2). Initially expressed as an inactive precursor, UFM1 undergoes proteolytic cleavage at the C-terminus to expose a conserved glycine residue that is necessary for UFM1 conjugation to target proteins. Conjugation of UFM1 to target proteins requires a UFM1-activating (E1) enzyme, a UFM1-conjugating (E2) enzyme, and a UFM1 ligase (E3) (1,3). UFM1 has been shown to be a mediator of ER stress-induced apoptosis and to promote the pathogenesis of Leishmania (2,4). This protein is modified with rhodamine red via primary amine coupling, resulting in the modification of lysine residues as well as the N-terminus. This labeled Ufm1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6-tag.
- Komatsu, M. et al. (2004) EMBO J. 23:1977.
- Lemaire, K. et al. (2011) PLoS One 6:e18517.
- Tatsumi, K. et al. (2010) J. Biol. Chem. 285:5417.
- Gannavaram, S. et al. (2012) Mol. Microbiol. 86:187.
Citation for Recombinant Human UFM1 Rhodamine Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Characterization of the deubiquitination activity and substrate specificity of the chicken ubiquitin-specific protease 1/USP associated factor 1 complex
Authors: H Zheng, M Wang, C Zhao, S Wu, P Yu, Y Lü, T Wang, Y Ai
PLoS ONE, 2017;12(11):e0186535.
Sample Types: Recombinant Protein
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