Recombinant Human UNC5H1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
6767-UN-050
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Recombinant Human UNC5H1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human Netrin-1 (Catalog # 6419-N1) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human UNC5H1 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.3‑1.5 μg/mL.

Source
Mouse myeloma cell line, NS0-derived human UNC5H1 protein
Human UNC5H1
(Gln26 - Tyr306)
Accession # NP_588610
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln26 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
57.9 kDa (monomer)
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6767-UN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UNC5H1

UNC5H1, also known as UNC5A, is a member of the UNC5 (Drosophila uncoordinated-5 homology) family of type I transmembrane proteins within the immunoglobulin (Ig) superfamily. UNC5H1-4 (UNC5A-D, respectively) have two Ig and one or two thrombospondin type 1 (TSP1) domains in their extracellular regions, and a ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). Human UNC5H1 cDNA encodes 842 amino acids (aa) including a 25 aa signal peptide, a 281 aa extracellular domain with one TSP1 domain, and a 515 aa cytoplasmic domain. Within the extracellular domain, human UNC5A shares approximately 99%, 99%, 98%, 98%, 97% and 96% aa sequence identity with mouse, equine, rat, canine, bovine and porcine one-TSP1 isoforms of UNC5H1. UNC5 proteins are receptors for the netrin/UNC6 family of laminin-related secreted axon guidance cues; UNC5H1 has been shown to bind netrin 1, 3 and 4 (2 ‑ 4). Netrins can act as a chemoattractant for some axons in the presence of DCC (Deleted in Colorectal Cancer) proteins. However, UNC5H1 association with netrins changes them to chemorepellents (1, 5, 6). UNC5H1 is expressed after developmental migration of motor neurons, presumably halting their migration (7). UNC5H1 can be removed from the cell surface via endocytosis following phosphorylation by protein kinase C (PKC) in complex with PICK1. In turn, PKC activity is regulated by the neuronal G protein-coupled adenosine receptor A2b R (6, 8, 9). The UNC5 and DCC families also act as dependence receptors, and are pro-apoptotic in the absence of netrins (10 ‑ 13). UNC5H1 transcription is enhanced by the tumor suppressor p53, allowing increased apoptosis of tumor cells in the absence of netrin and the presence of NRAGE (13, 14).

References
  1. Moore, S.W. et al. (2007) Adv. Exp. Med. Biol. 621:17.
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Wang, H. et al. (1999) J. Neurosci. 19:4938.
  4. Qin, S. et al. (2007) Mol. Cell. Neurosci. 34:243.
  5. Hong, K. et al. (1999) Cell 97:927.
  6. Bartoe, J.L. et al. (2006) J. Neurosci. 26:3192.
  7. Barrett, C. and S. Guthrie (2001) Mech. Dev. 106:163.
  8. Williams, M.E. et al. (2003) J. Neurosci. 23:11279.
  9. McKenna, W.L. et al. (2008) J. Neurochem. 104:1081.
  10. Llambi, F. et al. (2001) EMBO J. 20:2715.
  11. Thiebault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
  12. Williams, M.E. et al. (2006) Nat. Neurosci. 9:996.
  13. Miyamoto, Y. et al. (2010) Int. J. Oncol. 36:1253.
  14. Williams, M.E. et al. (2003) J. Biol. Chem. 278:17483.
Long Name
Netrin Receptor UNC5A
Entrez Gene IDs
90249 (Human); 60629 (Rat)
Alternate Names
KIAA1976netrin receptor UNC5A; netrin receptor Unc5h1; Protein unc-5 homolog 1; Protein unc-5 homolog A; unc5 (C.elegans homolog) a; unc-5 homolog 1; unc-5 homolog A (C. elegans); UNC5A; UNC5H1; UNC5H1FLJ16449

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