Recombinant Human UPP1 Protein, CF

R&D Systems | Catalog # 7234-UP

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human UPP1 Protein (7234-UP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q16831

Applications

Enzyme Activity
View all Uridine Phosphorylase 1/UPP1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Uridine Phosphorylase 1/UPP1 protein
Ala2-Ala310, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

35 kDa

SDS-PAGE

32-34 kDa, reducing conditions

Activity

Measured by its ability to catalyze the phosphorolysis of uridine to uracil and ribose-1-phosphate.
The specific activity is >9,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

7234-UP
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Uridine Phosphorylase 1/UPP1

Uridine Phophorylase 1 (UPP1) belongs to the family of pentosyltransferases. UPP1 is expressed in the cytosol of most tissues and at elevated levels in many tumors (1, 2). It plays an important role in the pyrimidine salvage pathway through its catalysis of the reversible phosphorolysis of uridine to uracil (1, 2). UPP1 is important for the homeostatic regulation of intracellular and plasma uridine concentrations (3). UPP1 also plays an important role in the activation of 5-fluorouracil, an anti-tumor drug (4). The high level of UPP1 expression in some tumors makes it a potential prognostic factor for some cancers, such as oral squamous cell carcinoma (5).

References

  1. Krenitsky, T. A. et al. (1964) J. Biol. Chem. 239:805.
  2. Cass, C. E. and Paterson, A. R. P. (1972) J. Biol. Chem. 247:3314.
  3. Connolly, G.P. and Duley, J. A. (1999) Trends Pharmacol. Sci. 20:218.
  4. Cao, D. and Pizzorno, G. (2004) Drugs Today (Barc) 40:431.
  5. Miyashita, H. et al. (2002) Cancer 94:2959.

Alternate Names

UDPRASE, UP, UPASE 1, UPP, UrdPase 1

Entrez Gene IDs

7378 (Human); 22271 (Mouse); 289801 (Rat)

Gene Symbol

UPP1

UniProt

Q16831

Additional Uridine Phosphorylase 1/UPP1 Products

Product Documents for Recombinant Human UPP1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human UPP1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Related Research Areas

Glycobiology-related Enzymes

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Protocols

View specific protocols for Recombinant Human UPP1 Protein, CF (7234-UP):

Materials
  • Assay Buffer: 0.1 M Potassium Phosphate, pH 7.4
  • Recombinant Human Uridine Phosphorylase 1/UPP1 (rhUPP1) (Catalog # 7234-UP)
  • Substrate: Uridine (Sigma, Catalog # U3003), 100 mM stock in deionized water
  • UV plate (Costar, Catalog # 3635)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhUPP1 to 2 ng/μL in Assay Buffer.
  2. Dilute Substrate to 2 mM in Assay Buffer.
  3. Load into a plate 50 μL of 2 ng/μL rhUPP1, and start the reaction by adding 50 μL of 2 mM Substrate. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of 2 mM Substrate.
  4. Read plate at a wavelength of 280 nm (bottom read) in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x -1 x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 1370 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhUPP1: 0.1 μg
  • Substrate: 1 mM

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