Recombinant Human URM1 Isoform 1 Protein, CF

Catalog # Availability Size / Price Qty
UL-950-250
Product Details
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Recombinant Human URM1 Isoform 1 Protein, CF Summary

Source
E. coli-derived human URM1 protein
Accession #
Structure / Form
Store at -80°C. Avoid multiple freeze/thaw cycles.

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-950

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: URM1

Ubiquitin Related Modifier 1 (URM1) is a conserved, ubiquitously expressed cytoplasmic Ubiquitin-like protein (1). It is 101 amino acids (aa) in length with a predicted molecular weight of 11.4 kDa (1,2). Human URM1 shares 93% and 94% aa sequence identity with the mouse and rat orthologs, respectively. URM1 contains a core beta -grasp fold and a C-terminal glycine-glycine motif characteristic of Ubiquitin and Ubiquitin-like modifiers (1,2). URM1 is activated by the Ubiquitin-activating (E1)-like enzyme MOCS3, Uba4 in yeast, via the formation of a thiocarboxylate group at its C-terminus. More specifically, MOCS3 and Uba4 mediate the adenylation and subsequent addition of sulfur to the carboxyl group of the C-terminal glycine residue of URM1 (3-5). Thiocarboxylated URM1 functions as a sulfur carrier in the thiolation of eukaryotic cytoplasmic transfer RNA (6-8). URM1 can also be conjugated to lysine residues of several proteins including yeast Ahp1 and human MOCS3, CTU1, and CTU2 (4,7,9). URM1 has been implicated in oxygen stress tolerance and nutrient sensing, invasive growth, and budding in yeast (4,9,10). This recombinant human protein is untagged. 

References
  1. Furukawa, K. et al. (2000) J. Biol. Chem. 275:7462.
  2. Singh, S. et al. (2005) Protein Sci. 14:2095.
  3. Schmitz, J. et al. (2008) Biochemistry 47:6479.
  4. Van der Veen, A.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1763.
  5. Chowdhury, M.M. et al. (2012) J. Biol. Chem. 287:17297.
  6. Schlieker, C.D. et al. (2008) Proc. Natl. Acad. Sci. USA 105:18255.
  7. Pedrioli, P.G. et al. (2008) EMBO Rep. 9:1196.
  8. Leidel, S. et al. (2009) Nature 458:228.
  9. Goehring, A.S. et al. (2003) Eukaryot. Cell 2:930.
  10. Goehring, A.S. et al. (2003) Mol. Biol. Cell 14:4329.
Long Name
Ubiquitin Related Modifier 1
Entrez Gene IDs
81605 (Human); 68205 (Mouse)
Alternate Names
C9orf74; chromosome 9 open reading frame 74; MGC2668; ubiquitin related modifier 1 homolog (S. cerevisiae); ubiquitin related modifier 1 homolog; ubiquitin related modifier 1; ubiquitin-related modifier 1 homolog; URM1

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