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Recombinant Human USP14 Protein, CF

Catalog #: E-544
3 Citations Datasheet / COA / SDS

Discontinued Product

E-544 has been discontinued.
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Recombinant Human USP14 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human USP14 is a Ubiquitin-specific deconjugating enzyme. The activity of Recombinant Human USP14 was measured at 2.0 RFU/min/μg using an enzyme concentration of 18 μM and a Ub-AMC concentration of 4 μM in 100 mM HEPES, pH 8.5, 10 mM DTT, and 5% Glycerol at 25 °C.  USP14 activity is greatly stimulated in the presence of 19S proteasome.
Source
E. coli-derived human USP14 protein
Accession #
P54578
Predicted Molecular Mass
56 kDa

Product Datasheets

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E-544

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-544

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 5 mM DTT, 1 mM EDTA
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP14

Ubiquitin Specific Peptidase 14 (USP14) is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes. It has a predicted molecular weight of 56.1 kDa. Human USP14 is 494 amino acids (aa) in length, contains an N-terminal Ubiquitin-like (Ubl) domain (4-80 aa), and shares 97% aa sequence identity with the mouse and rat orthologs (1,2). USP14 is one of three deubiquitinating enzymes associated with the 26S Proteasome (2-5). It reversibly binds the 19S regulatory particle via its Ubl domain and mediates the disassembly of Ubiquitin chains on substrates by the stepwise removal of Ubiquitin molecules from the distal tip of the chain (2,4,5). USP14 has also been shown to deubiquitinate the chemokine receptor CXCR4, regulating both CXCL12/SDF-1-induced chemotaxis and receptor degradation (6). USP14 appears to be critical for the development and function of neuromuscular junctions (7-9). Additionally, USP14 is thought to serve as a physiological inhibitor of endoplasmic reticulum-associated degradation under non-stressed conditions by inhibiting the degradation of unfolded endoplasmic reticulum proteins (10).

References
  1. Wyndham, A.M. et al. (1999) Protein Sci. 8:1268.
  2. Hu, M. et al. (2005) EMBO J. 24:3747.
  3. Borodovsky, A. et al. (2001) EMBO J. 20:5187.
  4. Koulich, E. et al. (2008) Mol. Biol. Cell 19:1072.
  5. Lee, M.J. et al. (2011) Mol. Cell. Proteomics 10:R110.003871.
  6. Mines, M.A. et al. (2009) J. Biol. Chem. 284:5742.
  7. Wilson, S.M. et al. (2002) Nat. Genet. 32:420.
  8. Chen, P.C. et al. (2009) J. Neurosci. 29:10909.
  9. Bhattacharyya, B.J. et al. (2012) Am. J. Physiol. Cell Physiol. 302:C698.
  10. Nagai, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:995.
Long Name
Ubiquitin Specific Protease 14
Entrez Gene IDs
9097 (Human); 59025 (Mouse); 291796 (Rat)
Alternate Names
Deubiquitinating enzyme 14; EC 3.1.2.15; EC 3.4.19.12; TGT; TGTubiquitin carboxyl-terminal hydrolase 14; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA-guanine transglycosylase); Ubiquitin Thioesterase 14; Ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14; Ubp6; USP14

Citations for Recombinant Human USP14 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1
    Authors: LD Harris, J Le Pen, N Scholz, J Mieszczane, N Vaughan, S Davis, G Berridge, BM Kessler, M Bienz, JDF Licchesi
    The Journal of Biological Chemistry, 2021-01-08;296(0):100246.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Typically inhibiting USP14 promotes autophagy in M1-like macrophages and alleviates CLP-induced sepsis
    Authors: F Xu, Y Ma, W Huang, J Gao, M Guo, J Li, L Kong, G Liang, R Du, Q Xu, X Wu
    Cell Death Dis, 2020-08-20;11(8):666.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. USP14 is a deubiquitinase for Ku70 and critical determinant of non-homologous end joining repair in autophagy and PTEN-deficient cells
    Authors: A Sharma, T Alswillah, I Kapoor, P Debjani, B Willard, MK Summers, Z Gong, A Almasan
    Nucleic Acids Res., 2020-01-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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