Recombinant Human Vitronectin Protein, Animal-Free

R&D Systems | Catalog # BT-VTN-AFL

N-terminal Variant
R&D Systems
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Key Product Details

Source

E. coli

Accession Number

P04004.1

Applications

Bioactivity
View all Vitronectin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Vitronectin protein
Val62-Glu292 with a C-terminal 6-His tag
Produced using non-animal reagents in an animal-free laboratory.

Purity

>90%, by HPLC.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Val62

Predicted Molecular Mass

27 kDa

Activity

Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells.
The ED50 for this effect is 10.0-120 ng/mL.

Scientific Data Images for Recombinant Human Vitronectin Protein, Animal-Free

Animal-Free™ Recombinant Human Vitronectin Protein Bioactivity.

Animal-Free™ Recombinant Human Vitronectin (Catalog # BT-VTN-AFL) supports the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 10.0-120 ng/mL.

Formulation, Preparation, and Storage

BT-VTN-AFL
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and TCEP with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vitronectin

Vitronectin is a large glycoprotein found in blood and the extracellular matrix (ECM). As one of the major cell adhesion molecules in plasma, it interacts with integrins in the extracellular environment. Due to its many functions for cell biology including supporting the growth, maintenance and differentiation of stem cells, Vitronectin is a crucial molecule in stem cell therapy applications.

A truncated, E. coli derived version of human Vitronectin (aa62-292) has been optimized for large scale stem cell culture applications. This Vitronectin contains the RGD sequence necessary for interaction with cell-surface receptors to maintain its ability to act as a stem cell substrate.

References

  1. Lu, X. et al. (2024) Cells 13:1566.

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Entrez Gene IDs

7448 (Human); 22370 (Mouse); 507525 (Bovine)

Gene Symbol

VTN

UniProt

P04004.1

Additional Vitronectin Products

Product Documents for Recombinant Human Vitronectin Protein, Animal-Free

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human Vitronectin Protein, Animal-Free

    For research use only

    Related Research Areas

    Extracellular Matrix Molecules

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