Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF

R&D Systems | Catalog # BT9200

R&D Systems
Discontinued Product
BT9200 has been discontinued. View all VSIG8 products.

Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer. Biotinylated via sugars

Conjugate

Biotin

Applications

Bioactivity
View all VSIG8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VSIG8 protein
Human VSIG8
(Val22-Gly263)
Accession # Q5VU13		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val22

Predicted Molecular Mass

54 kDa (unlabeled)

SDS-PAGE

56-67 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 1-5 μg/mL.

Scientific Data Images for Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF

Recombinant Human VSIG8 Fc Chimera Biotinylated Protein Bioactivity

Recombinant Human VSIG8 Fc Chimera Biotinylated Protein Bioactivity

Both Biotinylated Recombinant Human VSIG8 Fc Chimera (Catalog # BT9200) and Recombinant Human VSIG8 Fc Chimera (Catalog # 9200-VS) inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-5 µg/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation, and Storage

BT9200
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and Mannitol with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

Additional VSIG8 Products

Product Documents for Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF

For research use only

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