Recombinant Human WIF-1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
1341-WF-050/CF

With Carrier

Catalog # Availability Size / Price Qty
1341-WF-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (15)
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Recombinant Human WIF-1 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.3-1.5 µg/mL in the presence of 10 ng/mL of Wnt-3a.
Source
Mouse myeloma cell line, NS0-derived human WIF-1 protein
Gly29-Trp379, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Gly29
Predicted Molecular Mass
39.8 kDa
SDS-PAGE
50 kDa, reducing conditions

Product Datasheets

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1341-WF (with carrier)

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1341-WF/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1341-WF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid, NaCl, PEG3350 and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1341-WF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid, NaCl, PEG3350 and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: WIF-1

WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (AA) molecule that contains an N-terminal signal sequence, 150 AA WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus (1). In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles (1). WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos (1). In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt8 proteins (1). WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder (2). However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis (3). Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.

References
  1. Hsieh, J-C. et al. (1999) Nature 398:431.
  2. Wissmann, C. et al. (2003) J. Pathol 201:204.
  3. Cebrat, M. et al. (2004) Cancer Lett. 206:107.
Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

Citations for Recombinant Human WIF-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Tumor Explants Elucidate a Cascade of Paracrine SHH, WNT, and VEGF Signals Driving Pancreatic Cancer Angiosuppression
    Authors: Hasselluhn, MC;Decker-Farrell, AR;Vlahos, L;Thomas, DH;Curiel-Garcia, A;Maurer, HC;Wasko, UN;Tomassoni, L;Sastra, SA;Palermo, CF;Dalton, TC;Ma, A;Li, F;Tolosa, EJ;Hibshoosh, H;Fernandez-Zapico, ME;Muir, A;Califano, A;Olive, KP;
    Cancer discovery
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. Delivery of Wnt inhibitor WIF1 via engineered polymeric microspheres promotes nerve regeneration after sciatic nerve crush
    Authors: N Zhang, J Lin, JS Chin, C Wiraja, C Xu, DA McGrouther, SY Chew
    Journal of tissue engineering, 2022-04-07;13(0):2041731422108.
    Species: Rat
    Sample Types: Whole Cell
    Applications: Cell Culture
  3. Wnt Inhibitory Factor 1 Binds to and Inhibits the Activity of Sonic Hedgehog
    Authors: K Kerekes, M Trexler, L Bányai, L Patthy
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Protein
    Applications: Pull-Down Assay
  4. Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids
    Authors: G Pettinato, MF Coughlan, X Zhang, L Chen, U Khan, M Glyavina, CJ Sheil, PK Upputuri, YN Zakharov, E Vitkin, AB D'Assoro, RA Fisher, I Itzkan, L Zhang, L Qiu, LT Perelman
    Science Advances, 2021-08-18;7(34):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells
    Authors: G Pettinato, S Lehoux, R Ramanathan, MM Salem, LX He, O Muse, R Flaumenhaf, MT Thompson, EA Rouse, RD Cummings, X Wen, RA Fisher
    Sci Rep, 2019-06-20;9(1):8920.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  6. Tumor Suppressive Role of ARHGAP17 in Colon Cancer Through Wnt/?-Catenin Signaling
    Authors: S Pan, Y Deng, J Fu, Y Zhang, Z Zhang, X Ru, X Qin
    Cell. Physiol. Biochem., 2018-04-28;46(5):2138-2148.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. WIF1 prevents Wnt5A mediated LIMK/CFL phosphorylation and adherens junction disruption in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    J Inflamm (Lond), 2017-05-19;14(0):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Scalable Differentiation of Human iPSCs in a Multicellular Spheroid-based 3D Culture into Hepatocyte-like Cells through Direct Wnt/?-catenin Pathway Inhibition
    Sci Rep, 2016-09-12;6(0):32888.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling
    PLoS ONE, 2016-05-23;11(5):e0156002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    Cell Adh Migr, 2016-05-09;11(1):24-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.
    Authors: Tsakiridis, Anestis, Huang, Yali, Blin, Guillaum, Skylaki, Stavroul, Wymeersch, Filip, Osorno, Rodrigo, Economou, Costas, Karagianni, Eleni, Zhao, Suling, Lowell, Sally, Wilson, Valerie
    Development, 2014-03-01;141(6):1209-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Wnt inhibitory factor 1 (Wif1) is regulated by androgens and enhances androgen-dependent prostate development.
    Authors: Keil K, Mehta V, Branam A, Abler L, Buresh-Stiemke R, Joshi P, Schmitz C, Marker P, Vezina C
    Endocrinology, 2012-10-18;153(12):6091-103.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010-04-08;130(7):1849-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells.
    Authors: Cho SW, Yang JY, Sun HJ, Jung JY, Her SJ, Cho HY, Choi HJ, Kim SW, Kim SY, Shin CS
    Bone, 2009-02-27;44(6):1069-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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