Recombinant MERS-CoV 3CL Protease, CF New
Recombinant MERS-CoV 3CL Protease, CF Summary
For other Coronavirus proteases and recombinant proteins
Ser3248 - Gln3553
Contains the following substitutions: Val 3551 to Leu; Met 3552 to Phe
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||1.7 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM DTT|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: 3CL Protease
The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human MERS-CoV coronavirus (Middle East Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. 3CL protease activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid. This recombinant protein is untagged.
- Lu R. et al. (2015) Microbio.Res. Announc. doi 10.1128/genomeA.00818-15
- Tomar S. et al. (2015) J. Biol. Chem. 10.1074/jbc.M115.651463
- Wu A. et al. (2015) Virus Res. doi: 10.1016/j.virusres.2015.05.018
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