Recombinant MERS-CoV 3CL Protease, CF

R&D Systems | Catalog # E-719

R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant MERS-CoV 3CL Protease (E-719)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

YP_009047217.1

Applications

Bioactivity
View all 3CL Protease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mers-cov 3CL Protease protein
Ser1 - Gln306 (Val304Leu & Met305Phe) with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

33 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial MERS-CoV 3CL Protease concentration of 50-100 nM when using SARS CoV-2 3CL Protease Substrate, Rh110-conjugated (Catalog # S-720).

Formulation, Preparation, and Storage

E-719
Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: 3CL Protease

The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human MERS-CoV coronavirus (Middle East Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide that cleaves proteins with sequences including a LQ[S/A/G] c-terminal to the glutamine residue.  3CL protease activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  

References

  1. Lu R. et al. (2015) Genome Announc. 3:e00818.
  2. Tomar S. et al. (2015) J. Biol. Chem. 290:19403.
  3. Wu A. et al. (2015) Virus Res. 208:56.

Long Name

3CL Protease

Alternate Names

Main Protease, Mpro

UniProt

YP_009047217.1

Additional 3CL Protease Products

Product Documents for Recombinant MERS-CoV 3CL Protease, CF

Certificate of Analysis

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Product Specific Notices for Recombinant MERS-CoV 3CL Protease, CF

For research use only

Related Research Areas

Infectious Diseases

Citations for Recombinant MERS-CoV 3CL Protease, CF

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