Recombinant MERS-CoV His6 Papain-like Protease Protein, CF
Recombinant MERS-CoV His6 Papain-like Protease Protein, CF Summary
Leu1541 - Ser1859
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Papain-like Protease
The Papain-like protease ("PLPro") from the human Middle East Respiratory Syndrome (MERS) virus is essential for viral replication. In addition to cleaving the replicase polyprotein at sites located at the N-terminus of the replicase polyprotein, PLPro possesses both deubiquitinating and deISGylating activity and is capable of hydrolyzing polyubiquitin chains. PLPro antagonizes the induction of type I interferon signaling by clocking the phosphorylation and subsequent nuclear translocation of IRF3; it also prevents host NF kappa B signaling. MERS PLPro will hydrolyze K6-, K11-, K33-, K48- and K63-linked tetraubiquitin chains in vitro, and will also hydrolyze Ubiquitin-Rh110. This recombinant protein contains an N-terminal 6-His tag.
Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF (Catalog # E-609)?
Recombinant MERS-CoV His6 Papain-like Protease Protein, CF (Catalog # E-609) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for Catago #E-609 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).
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