Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

R&D Systems | Catalog # E-609

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Key Product Details

  • R&D Systems E. coli-derived Recombinant MERS-CoV His6 Papain-like Protease Protein (E-609)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

K9N638.1

Applications

Bioactivity
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Product Specifications

Source

E. coli-derived mers-cov Papain-like Protease protein
Leu1541 - Ser1859 with a N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

37 kDa

Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rh110 (U-555) substrate and 100-500 nM when using tetraubiquitin substrate.

Formulation, Preparation, and Storage

E-609
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Papain-like Protease

The Papain-like protease ("PLPro") from the human Middle East Respiratory Syndrome (MERS) virus is essential for viral replication.  In addition to cleaving the replicase polyprotein at sites located at the N-terminus of the replicase polyprotein, PLPro possesses both deubiquitinating and deISGylating activity and is capable of hydrolyzing polyubiquitin chains.  PLPro antagonizes the induction of type I interferon signaling by clocking the phosphorylation and subsequent nuclear translocation of IRF3; it also prevents host NF kappa B signaling.  MERS PLPro will hydrolyze K6-, K11-, K33-, K48- and K63-linked tetraubiquitin chains in vitro, and will also hydrolyze Ubiquitin-Rh110.  

References

  1. Clasman J.R. et al. (2020) Antiviral Res. 174:104661.

Alternate Names

PL-PRO, PLpro, pp1a, Replicase polyprotein 1a

UniProt

K9N638.1

Additional Papain-like Protease Products

Product Documents for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

For research use only

Citations for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

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FAQs for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

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  • Q: Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF (Catalog # E-609)?

    A: Recombinant MERS-CoV His6 Papain-like Protease Protein, CF (Catalog # E-609) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for Catago #E-609 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).

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