Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF

R&D Systems | Catalog # 11279-MX

Analyzed by SEC-MALS
R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Monkeypox Virus A30L Fc Chimera Protein (11279-MX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_536567.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all A30L Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A30L protein
MD Human IgG1
(Pro100-Lys330)		 IEGR
 MPXV A30L
(Gln22-Leu146)
Accession # NP_536567.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

41 kDa

SDS-PAGE

52-60 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 A30L Fc Chimera Protein SEC-MALS.

Recombinant Monkeypox Virus Zaire-96-I-16 A30L Fc Chimera Protein (Catalog # 11279-MX) has a molecular weight (MW) of 95.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Monkeypox Virus Zaire-96-I-16 A30L Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 A30L Fc Chimera Protein (Catalog # 11279-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-60 kDa and 100-120 kDa, respectively.

Formulation, Preparation, and Storage

11279-MX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: A30L

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L,~14 kDa and H3L, ~32kDa), an envelope glycoprotein (A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3). The envelope protein A30L was reported to play a role in association of viral membrane with the dense viroplasm (4).

References

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Szajner, P. et al. (2006) J. Virol. 80:306.

Long Name

Envelope Protein A28 Homolog

Entrez Gene IDs

928965 (Monkeypox Virus Zaire-96-I-16)

Gene Symbol

OPG155

UniProt

NP_536567.1

Additional A30L Products

Product Documents for Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF

For research use only

Customer Reviews for Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes