Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF
Recombinant Monkeypox Virus A30L Fc Chimera Protein, CF Summary
- R&D Systems HEK293-derived Recombinant Monkeypox Virus A30L Fc Chimera Protein (11279-MX)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) | IEGR | MPXV A30L (Gln22-Leu146) Accession # NP_536567.1 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11279-MX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Monkeypox Virus Zaire-96-I-16 A30L Fc Chimera Protein (Catalog # 11279-MX) has a molecular weight (MW) of 95.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).
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2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 A30L Fc Chimera Protein (Catalog # 11279-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-60 kDa and 100-120 kDa, respectively.
Reconstitution Calculator
Background: A30L
Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L,~14 kDa and H3L, ~32kDa), an envelope glycoprotein (A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3). The envelope protein A30L was reported to play a role in association of viral membrane with the dense viroplasm (4).
- Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
- Farahat, R.A. et al. (2022) Infez Med. 30:372.
- Schelkunov, S.N. et al. (2002) Virology 297:172.
- Szajner, P. et al. (2006) J. Virol. 80:306.
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