Recombinant Monkeypox Virus A35R Fc Chimera Protein, CF

R&D Systems | Catalog # 11278-MX

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Monkeypox Virus A35R Fc Chimera Protein (11278-MX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_536572.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all A35R Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A35R protein
MD Human IgG1
(Pro100-Lys330)		 IEGR MPXV A35R
(Arg58-Thr181)
Accession # NP_536572.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

40 kDa

SDS-PAGE

41-47 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Monkeypox Virus A35R Fc Chimera Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 A35R Fc Chimera Protein SEC-MALS.

Recombinant Monkeypox Virus Zaire-96-I-16 A35R Fc Chimera Protein (Catalog # 11278-MX) has a molecular weight (MW) of 84.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Monkeypox Virus Zaire-96-I-16 A35R Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 A35R Fc Chimera Protein (Catalog # 11278-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-47 kDa and 80-100 kDa, respectively.

Formulation, Preparation, and Storage

11278-MX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: A35R

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L,~14 kDa and H3L, ~32kDa), an envelope glycoprotein (A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3). The envelope glycoprotein A35R is highly conserved among poxviruses. It is not required for replication, but it was reported  to play a role in virulence (4). In addition, A35R was showed to inhibit MHC class II antigen presentation (5).

References

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Roper, R. L. (2006) J. Virol. 80:306.
  5. Rehm, K. E. et al. (2010) Virology 397:176.

Long Name

EEV Envelope Glycoprotein

Entrez Gene IDs

928958 (Monkeypox Virus Zaire-96-I-16)

Gene Symbol

OPG161

UniProt

NP_536572.1

Additional A35R Products

Product Documents for Recombinant Monkeypox Virus A35R Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Monkeypox Virus A35R Fc Chimera Protein, CF

For research use only

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