Recombinant Monkeypox Virus A35R Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Monkeypox Virus Zaire-96-I-16 A35R Fc Chimera Protein SDS-PAGE.
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Product Details

Recombinant Monkeypox Virus A35R Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Bioassay data are not available.
Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A35R protein
MDHuman IgG1
Accession # NP_536572.1
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa
41-47 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 A35R Fc Chimera Protein (Catalog # 11278-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-47 kDa and 80-100 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L,~14 kDa and H3L, ~32kDa), an envelope glycoprotein (A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3). The envelope glycoprotein A35R is highly conserved among poxviruses. It is not required for replication, but it was reported  to play a role in virulence (4). In addition, A35R was showed to inhibit MHC class II antigen presentation (5).

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Roper, R. L. (2006) J. Virol. 80:306.
  5. Rehm, K. E. et al. (2010) Virology 397:176.


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