Recombinant Mouse Activin B Protein, CF

(2 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2‑1.2 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Gly297-Ala411
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly297
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    13 kDa (monomer)
  • SDS-PAGE
    12 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8260-AB/CF
 
8260-AB
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Activin B (Catalog # 8260-AB/CF) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.
1 μg/lane ofRecombinant Mouse Activin Bwas resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 12 and 21 kDa, respectively.
Background: Activin B
Activin and Inhibin, members of the TGF-beta superfamily of cytokines, are involved in a range of biological processes including neural development, stem cell differentiation, reproductive physiology, inflammation, tissue morphogenesis and bone remodeling (1-5). Activins function as either a homodimer or heterodimer of non-glycosylated beta subunit proteins ( beta A, beta B, beta C, and beta E in mammals). Inhibins are heterodimers consisting of a unique alpha  subunit and any one beta  subunit. The alpha - and beta -subunits are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release a carboxy-terminal monomeric subunit that becomes bioactive upon disulfide linkage with another Activin monomer (6, 7). The bioactive Activin B dimeric protein consists of two beta B subunits. The 13 kDa mature mouse Activin beta B subunit shares 99% and 98% amino acid sequence identity with rat and human Activin beta B, respectively. Activin B exerts its biological function upon binding to the type 2 serine/threonine kinase Activin receptor (Act RIIA). This receptor-ligand complex noncovalently associates and transphosphorylates the type 1 Activin receptor (ActRI) to initiate intracellular SMAD activation and the subsequent regulation of Activin-responsive gene transcription (8). BAMBI, Betaglycan, and Cripto, regulate the bioactivity of Activin B by restricting its ability to induce receptor complex assembly. Alternatively, bioavailability of Activin B is modulated through sequestration into inactive complexes with alpha 2-Macroglobulin, Follstatin, and FLRG (9-12).
  • References:
    1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
    2. Paulusma, C.C. et al. (1994) J. Immunol. Methods 169:143.
    3. Vassalli, A. et al. (1994) Genes Dev. 8:414.
    4. Shat-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
    5. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
    6. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
    7. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
    8. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
    9. Hedger, M.P. and D.M. de Kretser (2013) Cytokine Growth Factor Rev. 24: 285.
    10. Gray, P.C. and W. Vale (2012) FEBS Letters 586:1836.
    11. Kelber, J.A. et al. (2008) J. Biol. Chem. 283: 4490.
    12. Xia, Y. and A.L. Schneyer (2009) Journal of Endocrinology 202:1.
  • Entrez Gene IDs:
    3625 (Human); 16324 (Mouse)
  • Alternate Names:
    Activin B; INHBB; Inhibin, Beta B; Inhibin, Beta-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Activin Receptor Type IIA (ACVR2A) Functions Directly in Osteoblasts as a Negative Regulator of Bone Mass
    Authors: BC Goh, V Singhal, AJ Herrera, RE Tomlinson, S Kim, MC Faugere, EL Germain-Le, TL Clemens, SJ Lee, DJ DiGirolamo
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Adipocyte ALK7 links nutrient overload to catecholamine resistance in obesity.
    Authors: Guo T, Marmol P, Moliner A, Bjornholm M, Zhang C, Shokat K, Ibanez C
    Elife, 2014;3(0):e03245.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay

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