Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF

R&D Systems | Catalog # 2494-ZN

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein (2494-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q91Y31

Applications

Enzyme Activity
View all Aminopeptidase P2/XPNPEP2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Aminopeptidase P2/XPNPEP2 protein
Tyr22-Ala650 & Pro23-Ala650, both with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr22 & Pro23

Predicted Molecular Mass

73 kDa

SDS-PAGE

93 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)-Pro-Pro-p-Nitroanilide (K(Abz)PP-pNA).
The specific activity is >175 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

2494-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aminopeptidase P2/XPNPEP2

The mouse XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1, 2). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (3). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (4). Mouse APP2 is expressed in many adult tissues and in embryos (5). The purified rmAPP2 corresponds to the ectodomain and is shown here to be an active aminopeptidase, removing an N-terminal amino acid from a peptide that contains a Pro residue at the second position.

References

  1. Kawai, J. et al. (2001) Nature 409:685.
  2. Shibata, K. et al. (2000) Genome Res. 10:1757.
  3. Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.
  4. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego.
  5. Ersahin C. et al. (2003) Arch. Biochem. Biophys. 417:131.
 

Alternate Names

XPNPEP2

Entrez Gene IDs

7512 (Human); 170745 (Mouse)

Gene Symbol

XPNPEP2

UniProt

Q91Y31

Additional Aminopeptidase P2/XPNPEP2 Products

Product Documents for Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This Product is manufactured under a license to U.S. Patent Nos. 6,399,349 and 7,273,718.

Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.

For research use only

Related Research Areas

Metalloproteases and Regulators

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Protocols

View specific protocols for Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF (2494-ZN):

Materials
  • Assay Buffer: 100 mM HEPES, 0.5 mM MnCl2, pH 7.5
  • Recombinant Mouse Aminopeptidase P2/XPNPEP2 (rmXPNPEP) (Catalog # 2494-ZN)
  • Substrate: K(Abz)PP-pNA (Bachem, Catalog # L-1980)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: Spectramax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmXPNPEP2 to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 100 µM in Assay Buffer.
  3. In a plate load 50 µL of 2 ng/µL of rmXPNPEP2, and start the reaction by adding 50 µL of 100 µM Substrate to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 100 µM Substrate.
  4. Read at excitation and emission wavelengths of 320 nm and 410 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard Abz-Gly (Bachem, Catalog # E-2920).

Per Well:
  • rmXPNPEP2: 0.1 µg
  • Substrate: 50 µM

FAQs

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