Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF

R&D Systems | Catalog # 2500-ZN

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein (2500-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q6GTP5

Applications

Enzyme Activity
View all Aminopeptidase PILS/ARTS1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Aminopeptidase PILS/ARTS1 protein
Ser27-Leu930, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

105 kDa

SDS-PAGE

111 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 2500-ZN in the following applications:

Formulation, Preparation, and Storage

2500-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aminopeptidase PILS/ARTS1

The mouse ARTS1 gene encodes aminopeptidase PILS (Puromycin-Insensitive Leucyl-Specific), which is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum) (1-3). The deduced amino acid sequence of mouse ARTS1 consists of a signal peptide and a large ectodomain. Widely expressed and releasing an N‑terminal amino acid (Leu), the enzyme may play a role in many processes such as antigen processing and angiogenesis.

References

  1. Miyashita, H. et al. (2002) Blood 99:3241.
  2. Serwold, T. et al. (2002) Nature 419:480.
  3. Schomburg, L. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 311, Academic Press, San Diego.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Entrez Gene IDs

51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)

Gene Symbol

ERAP1

UniProt

Q6GTP5

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF

Customer Reviews for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF
    Name: Rachel Ngo
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 09/25/2020

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

View specific protocols for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF (2500-ZN):

Materials
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Mouse Aminopeptidase PILS/ARTS1 (rmARTS1) (Catalog # 2500-ZN)
  • Substrate: Leu-AMC (Bachem, Catalog # I-1240)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmARTS1 to 2 µg/mL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load 50 µL of the 2 µg/mL rmARTS1 into a plate, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin AMC (Sigma, Catalog # A-9891).

Per Well:
  • rmARTS1: 0.1 µg
  • Substrate: 100 µM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes