Recombinant Mouse ANGPTL8/Betatrophin His-tag Protein, CF

Mucin Stalk Chimera
Catalog # Availability Size / Price Qty
10194-AN-050
Recombinant Mouse ANGPTL8/Betatrophin His-tag Protein, CF Binding Activity
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Recombinant Mouse ANGPTL8/Betatrophin His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin Mucin stalk Chimera His-tag (Catalog # 10194-AN) that produces 50% of the optimal binding response is 12.5-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 8/Betatrophin protein
Mouse Angiopoietin-like Protein 8/Betatrophin
(Ala22-Ala198)
Accession # Q8R1L8

Human Fractalkine (Mucin Stalk)
(Phe103-Thr338)

HHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ala22
Predicted Molecular Mass
45 kDa
SDS-PAGE
74-90 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10194-AN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse ANGPTL8/Betatrophin Mucin Stalk Chimera His-tag (Catalog # 10194-AN) binds with an ED50 of 12.5‑100 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse ANGPTL8/Betatrophin Mucin Stalk Chimera His-tag (Catalog # 10194-AN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-90 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 8/Betatrophin

Betatrophin (also called ANGPTL8, lipasin and RIPL), a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. It is most homologous to ANGPTL3 but lacks the fibrinogen-like domain of ANGPTL3 and other ANGPTL family members (1). Mouse Betatrophin is 183 amino acids (aa) in length and shares 72% aa identity with human Betatrophin. Betatrophin is a recently identified hormone which has been associated with two functionally important processes in the development of type 2 diabetes and obesity, insulin resistance as well as being a crucial modulator in lipid metabolism through regulation of serum triglyceride levels (2, 3). Betatrophin can form a complex with the N-terminal of ANGPTL3, and this complex is necessary for inhibition of lipoprotein lipase (LPL) activity and triglyceride modulation (4). Elevated levels of circulating betatrophin in the blood may be associated with gestational diabetes mellitus (GDM) and polycystic ovary syndrome (PCOS) (5, 6). Increased levels of Betatrophin in plasma and adipose tissue may also be associated with hypertension, coronary artery disease (CAD), and peripheral artery disease (PAD) (7, 8). The role of Betatrophin in inducing pancreatic beta-cell proliferation is controversial, as it did not control beta-cell expansion in the mouse model (9, 10). In addition to the lipid and glucose metabolism, ANGPTL8 has been reported to be involved in many other disorders such as non-alcoholic fatty liver disease and renal dysfunction (11).

References
  1. Quagliarini, F. et al. (2012) PNAS 109:19751.
  2. Abu-Farha, M. et al. (2017) Diabetes Metab Res Rev. 33:e2919.
  3. Zhang, R. et al. (2012) Biochem Biophys Res Commun. 424:786.
  4. Chi, X. et al. (2017) Molecular Metabolism 6:1137.
  5. Kong, F.J. et al. (2017) PLoS One. 12:e0169941.
  6. Varikasuvu, S.R. et al. (2019) Gynecol Endocrinol. 35:190.
  7. Abu-Farha, M. et al. (2018) Lipids Health Dis. 17:35.
  8. Niki, H. et al. (2019) J Atheroscler Thromb. 26:doi:10.5551.
  9. Abu-Farha, M. et al. (2016) J Diabetes Res. 2016:4860595.
  10. Cox, A.R. et al. PLoS One (2016) 11:e0159276.
  11. Luo, M. et al. (2018) Front Endocrino 9:169.
Entrez Gene IDs
55908 (Human); 624219 (Mouse); 100361444 (Rat)
Alternate Names
Angiopoietin like Protein 8; Angiopoietin-like Protein 8; ANGPTL8; Betatrophin; C19orf80; chromosome 19 open reading frame 80; Gm6484; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; Lipasin; PRO1185; PVPA599; RIFL; TD26; UNQ599

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