ARSG is a member of the aryl sulfatase family of enzymes with a high degree of amino acid sequence similarity to ARSA and ARSB. ARSG mRNA is expressed in a ubiquitous manner, being detected in most adult and embryonic tissues of the mouse (1).
Recombinant Mouse Arylsulfatase G/ARSG Protein, CF
R&D Systems | Catalog # 4600-SU
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Key Product Details
- R&D Systems CHO-derived Recombinant Mouse Arylsulfatase G/ARSG Protein (4600-SU)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Arylsulfatase G/ARSG protein
Gly17-Val525, with a C-terminal 10-His tag
Accession # Q3TYD4
Gly17-Val525, with a C-terminal 10-His tag
Accession # Q3TYD4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly17
Predicted Molecular Mass
57 kDa
SDS-PAGE
65 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is > 1,500 pmol/min/µg, as measured under the described conditions.
The specific activity is > 1,500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
4600-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Arylsulfatase G/ARSG
References
- Ferrante, F. et al. (2002) Eur. J. Hum. Genetics 10:813.
Alternate Names
ARSG, ASG
Gene Symbol
ARSG
UniProt
Additional Arylsulfatase G/ARSG Products
Product Documents for Recombinant Mouse Arylsulfatase G/ARSG Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Arylsulfatase G/ARSG Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Mouse Arylsulfatase G/ARSG Protein, CF (4600-SU):
Materials
- Assay Buffer: 50 mM MES, 250 mM NaCl, pH 5.5
- Recombinant Mouse Arylsulfatase G/ARSG (rmARSG) (Catalog # 4600-SU)
- Substrate: 4-Nitrocatechol Sulfate (PNCS) (Sigma, Catalog # N-7251), 100 mM in deionized water
- Sodium Hydroxide, 1 M stock in deionized water
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rmARSG to 1 µg/mL in Assay Buffer.
- Dilute Substrate to 2 mM in Assay Buffer.
- Mix 75 μL of 1 µg/mL of rmARSG and 75 µL of 2 mM of Substrate in duplicate. Create a Substrate Blank with 75 µL Assay Buffer and 75 µL Substrate in duplicate. Incubate at 37 °C for 1 hour.
- Stop reaction by adding 150 µL of 0.2 M sodium hydroxide to the reaction vials.
- Load into a clear 96-well plate 200 µL from each reaction vial.
- Read at 510 nm (absorbance) in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Abs* (OD) x Conversion Factor** (pmol/OD) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard P-Nitrocatechol (PNC) (Sigma, Catalog # N15553).
- rmARSG: 0.05 µg
- Substrate: 0.5 mM
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