Recombinant Mouse BMPR-IB/ALK-6 Fc Chimera (NS0) Protein

  (3 citations)     
Datasheet / CoA / SDS
Product Details
Citations (3)
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.5-2 µg/mL in the presence of 30 ng/mL of rhBMP-4.
  • Source
    Mouse myeloma cell line, NS0-derived mouse BMPR-IB/ALK-6 protein
    Mouse BMPR-IB
    (Leu2 - Lys126)
    Accession # P36898
    DIEGRMD Human IgG1
    (Pro100 - Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu2
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    40.6 kDa (monomer)
  • SDS-PAGE
    55 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
444-BNS
 
444-BNS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity.

  • References:
    1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
    2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
  • Long Name:
    Bone Morphogenetic Protein Receptor IB
  • Entrez Gene IDs:
    658 (Human); 12167 (Mouse)
  • Alternate Names:
    activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. ALK1 signaling regulates early postnatal lymphatic vessel development.
    Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
    Blood, 2010;115(8):1654-61.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  2. PLAP-1/asporin inhibits activation of BMP receptor via its leucine-rich repeat motif.
    Authors: Tomoeda M, Yamada S, Shirai H, Ozawa Y, Yanagita M, Murakami S
    Biochem. Biophys. Res. Commun., 2008;371(2):191-6.
    Species: Human
    Sample Type: Complex Sample Type
    Application: IP
  3. Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling.
    Authors: Close JL, Gumuscu B, Reh TA
    Development, 2005;132(13):3015-26.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay

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Primary Antibodies
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Human IgG1 Antibody

WB , ELISA MAB9894  
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Human IgG1 Antibody

WB MAB98941  
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Recombinant Proteins
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Recombinant Human IgG1 Fc Protein, CF

BA 110-HG 72
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