Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

R&D Systems | Catalog # 6768-CA

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Cadherin-13 (CAD-13) Protein (6768-CA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_062681

Applications

Bioactivity
View all Cadherin-13 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cadherin-13 protein
Met1-Gly693, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

74.4 kDa

SDS-PAGE

100-120 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

6768-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Cadherin-13

Cadherin-13, also known as T-Cadherin and H‑Cadherin, is a GPI-anchored protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Following removal of its C‑terminal propeptide, Cadherin-13 is expressed on the cell surface both with and without its N-terminal propeptide in species of approximately 120 kDa and 100 kDa, respectively (3, 4). Mouse Cadherin-13 contains five tandem Cadherin repeats and shares 95% and 98% aa sequence identity with human and rat Cadherin-13, respectively (5). It is most highly expressed in the circulatory and nervous systems, particularly on cardiac myocytes, vascular endothelial and smooth muscle cells, and neurons and astrocytes in the brain (3, 4, 6 - 9). Cadherin-13 exerts a negative influence on neurite extension, and it is down‑regulated in neurons upon NGF stimulation (10, 11). Homotypic Cadherin-13 interactions promote intercellular adhesion which can be inhibited by its binding to LDL (12). Cadherin-13 also mediates the cardioprotective effects of Adiponectin by directly binding Adiponectin (selectively the hexameric and HMW forms) and trapping it on vascular endothelial cells and cardiomyocytes (6 ‑ 8). The role of Cadherin-13 in the vasculature is complex. When expressed on vascular endothelial cells it promotes angiogenesis, but when expressed on stromal cells it inhibits neovascularization (13, 14). Its down‑regulation on breast carcinoma cells and up‑regulation on the vasculature of various tumors limits tumor cell proliferation and angiogenesis but also enhances tumor progression (4, 8, 15).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.
  3. Stambolsky, D.V. et al. (1999) Biochim. Biophys. Acta 1416:155.
  4. Doyle, D.D. et al. (1998) J. Biol. Chem. 273:6937.
  5. Wyder, L. et al. (2000) Cancer Res. 60:4682.
  6. Denzel, M.S. et al. (2010) J. Clin. Invest. 120:4342.
  7. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  8. Hebbard, L.W. et al. (2008) Cancer Res. 68:1407.
  9. Takeuchi, T. et al. (2000) J. Neurochem. 74:1489.
  10. Fredette, B.J. et al. (1996) Development 122:3163.
  11. Bai, S. et al. (2007) J. Biol. Chem. 282:27171.
  12. Resink, T.J. et al. (1999) FEBS Lett. 463:29.
  13. Philippova, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2222.
  14. Rubina, K. et al. (2007) Angiogenesis 10:183.
  15. Lee, S.W. (1996) Nat. Med. 2:776.

Alternate Names

Cadherin13, CDH13, H-Cadherin, T-Cadherin

Entrez Gene IDs

1012 (Human); 12554 (Mouse); 192248 (Rat)

Gene Symbol

CDH13

UniProt

NP_062681

Additional Cadherin-13 Products

Product Documents for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

For research use only

Citations for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

Customer Reviews for Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF and earn rewards!

Have you used Recombinant Mouse Cadherin-13 (CAD-13) Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes