Recombinant Mouse CCL9/10/MIP-1 gamma (Truncated) Protein

Carrier Free

Catalog # Availability Size / Price Qty
3217-MG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3217-MG-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Recombinant Mouse CCL9/10/MIP-1 gamma (Truncated) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.2-1 ng/mL.
Source
E. coli-derived mouse CCL9/10/MIP-1 gamma protein
Gly50-Gln122
Accession #
N-terminal Sequence
Analysis
Gly50
Predicted Molecular Mass
8.4 kDa

Product Datasheets

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3217-MG (with carrier)

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3217-MG/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3217-MG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3217-MG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1 - 3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six-cysteine containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3 - 7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71 - 73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full-length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

References
  1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
  3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
  4. Haelens, A. et al. (1996) Immunobiology 195:499.
  5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
  6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
  7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.
Entrez Gene IDs
20308 (Mouse)
Alternate Names
CCF18; CCL9/10; MIP1 gamma; MIP-1 gamma

Citations for Recombinant Mouse CCL9/10/MIP-1 gamma (Truncated) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Chemokine CCL9 Is Upregulated Early in Chronic Kidney Disease and Counteracts Kidney Inflammation and Fibrosis
    Authors: C Hemmers, C Schulte, J Wollenhaup, DWL Wong, E Harlacher, S Orth-Alamp, BM Klinkhamme, SH Schirmer, M Böhm, N Marx, T Speer, P Boor, J Jankowski, H Noels
    Biomedicines, 2022;10(2):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  2. The HIV co-receptor CCR5 regulates osteoclast function
    Authors: JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura
    Nat Commun, 2017;8(1):2226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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