CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA‑derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).
Recombinant Mouse CD157 Protein, CF
R&D Systems | Catalog # 4710-AC
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse CD157 Protein (4710-AC)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CD157 protein
Ala25-Glu285, with a C-terminal 6-His tag
Ala25-Glu285, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala25
Predicted Molecular Mass
30 kDa
SDS-PAGE
37 kDa, 40 kDa and 44 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >10 pmol/min/µg, as measured under the described conditions.
The specific activity is >10 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
4710-AC
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD157
References
- Hussain, A. M. M. et al. (1998) Protein Express. Purif. 12:133.
- Sato, A. et al. (1999) Biochem. J. 337:491.
- Kaisho, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5325.
- Ortolan, E. et al. (2002) Cell Biochem. and Function. 20:309.
Long Name
Cluster of Differentiation 157
Alternate Names
BP-3, BST1, cADPr hydrolase 2, CD157
Gene Symbol
BST1
UniProt
Additional CD157 Products
Product Documents for Recombinant Mouse CD157 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse CD157 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Mouse CD157 Protein, CF (4710-AC):
Materials
- Assay Buffer: 50 mM MES, 2.5 mM ZnCl2, pH 6.5
- Recombinant Mouse CD157 (rmCD157) (Catalog # 4710-AC)
- Substrate: Nicotinamide guanine dinucleotide (NGD+) (Sigma, Catalog # N-5131), 10 mM stock in deionized water
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmCD157 to 5 µg/mL in Assay Buffer.
- Dilute Substrate to 200 µM in Assay Buffer.
- Into plate load 50 µL of 5 µg/mL rmCD157 and start the reaction by adding 50 µL of 200 µM Substrate. Include a blank control containing 50 µL Assay Buffer and 50 µL Substrate.
- Mix well.
- Incubate for 90 minutes at room temperature.
- Read at excitation and emission wavelengths of 300 nm and 410 nm (top read), respectively in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Fluorescence* (RFU) x Conversion Factor** (pmol/RFU) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard standard cyclic GDP ribose. Per Well:
- rmCD157: 0.25 µg
- Substrate: 100 µM