Recombinant Mouse CD177 Protein, CF

Catalog # Availability Size / Price Qty
8186-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CD177 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells. The ED50 for this effect is 0.2-1 μg/mL
Source
Mouse myeloma cell line, NS0-derived mouse CD177 protein
Leu22-Ala786, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu22
Predicted Molecular Mass
83 kDa
SDS-PAGE
100-115 kDa, reducing conditions

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8186-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8186-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD177

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References
  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
  3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  4. Klippel, S. et al. (2002) Blood 100:2441.
  5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
  6. Temerinac, S. et al. (2000) Blood 95:2569.
  7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
  8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
  9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
  10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
  11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
  12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
Long Name
Polycythemia Rubra Vera Protein 1
Entrez Gene IDs
57126 (Human); 68891 (Mouse)
Alternate Names
CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein

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