Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF

R&D Systems | Catalog # 7190-CD

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein (7190-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9Z1P5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD320/TCblR/8D6A Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD320/TCblR/8D6A protein
Mouse CD320
(Met1 - Gly208)
Accession # Q9Z1P5		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala29

Predicted Molecular Mass

46.7 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Activity

Measured by its ability to bind plasma Cobalamin (Cbl) bound Transcobalamin (TC). Quadros, E.V. et al. (2009) Blood 113:186.
When Recombinant Mouse CD320/TCblR/8D6A Fc Chimera is coated at 1 μg/mL (100 μL/well), it can bind human plasma TC (dilutions from 1:6 to 1:4000) in the presence of Cbl.

Formulation, Preparation, and Storage

7190-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD320/TCblR/8D6A

CD320, also known as the Transcobalamin II receptor (TCblR) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature mouse CD320 consists of a 180 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2, 3). Within the extracellular domain, mouse CD320 shares 52% aa sequence identity with human and rat CD320. Alternate splicing of mouse CD320 generates an additional isoform with a substitution of the N-terminal 40 aa. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B12) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a costimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).

References

  1. May, P. et al. (2007) Ann. Med. 39:219.
  2. Li, L. et al. (2000) J. Exp. Med. 191:1077.
  3. Quadros, E.V. et al. (2009) Blood 113:186.
  4. Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
  5. Park, H.J. et al. (2009) Immune Netw. 9:138.
  6. Cho, W. et al. (2008) BMC Rep. 41:863.
  7. Zhang, X. et al. (2001) J. Immunol. 167:49.
  8. Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
  9. Li, L. et al. (2004) Blood 104:815.

Long Name

Transcobalamin II Receptor

Alternate Names

8D6A, CD320, TCblR

Entrez Gene IDs

51293 (Human); 54219 (Mouse); 362851 (Rat)

Gene Symbol

CD320

UniProt

Q9Z1P5

Additional CD320/TCblR/8D6A Products

Product Documents for Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF

For research use only

Related Research Areas

Dendritic Cell Activation

Customer Reviews for Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes