Recombinant Mouse CD99 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
3905-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CD99 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind Recombinant Mouse PILR‑ alpha (Catalog # 4318-PR) in a functional ELISA. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Source
Mouse myeloma cell line, NS0-derived mouse CD99 protein
Mouse CD99
(Ala27 - Gly137)
Accession # AAH19482
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37.6 kDa (monomer)
SDS-PAGE
50-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3905-CD

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
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Background: CD99

Mouse CD99, also called PILR-L (paired immunoglobulin-like type 2 receptor ligand), is a 25 kDa type I transmembrane glycoprotein. The CD99 family of molecules also includes CD99-L2, Xga, and the pseudogene CD99L1 (1 - 4). The mouse CD99 cDNA encodes a 175 amino acid (aa) precursor with a 28 aa signal sequence, a 109 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 17 aa cytoplasmic region. The ECD contains no identifiable motifs, N-linked glycosylation sites, or cysteine residues, but has acidic, proline- and glycine-rich regions and sites for O-linked glycosylation that are conserved across species (4, 5). Mouse CD99 is thought to be rapidly evolving, and shares only 60% aa identity with rat, plus 40 - 48% aa identity with human, equine and canine CD99 (3, 5). The only known isoform of mouse CD99 appears to have a non-functional signal sequence (5). In contrast, human CD99 has long and short isoforms that show differences in function (6). Mouse CD99 may be either expressed on the surface, or retained within the cell as a homodimer, as found in naive CD4+ or CD8+ T cells (5, 7). CD99 is expressed by most leukocytes and vascular endothelial cells, especially at contact sites (3, 8). CD99 molecules on one cell can interact with CD99 on adjacent cells. CD99 can also interact with PILR-alpha and PILR-beta, which are expressed on granulocytes, macrophages, NK and dendritic cells (1, 9). The interactions regulate the transendothelial migration of lymphocytes and neutrophils to inflamed tissues (9). Sialylated sugars O-linked to CD99 at Thr45 and Thr50 mediate PILR recognition of CD99. Either is sufficient for inhibitory PILR-alpha recognition, while the Thr45 site is specifically required for activating PILR-beta recognition (10, 11).

References
  1. Wilson, M.D. et al. (2006) Physiol Genomics 27:201.
  2. Petri, B. and M.G. Bixel (2006) FEBS J. 273:4399.
  3. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
  4. Suh, Y.H. et al. (2003) Gene 307:63.
  5. Park, S.H. et al. (2005) Gene 353:177.
  6. Byun, H-J. et al. (2006) J. Biol. Chem. 281:34833.
  7. Choi, G. et al. (2007) Exp. Mol. Med. 39:746.
  8. Bixel, G. et al. (2004) Blood 104:3205.
  9. Bixel, G. et al. (2007) Blood 109:5327.
  10. Wang, J. et al. (2008) J. Immunol. 180:1686.
  11. Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
Entrez Gene IDs
4267 (Human); 673094 (Mouse); 652929 (Rat)
Alternate Names
12E7; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2

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