Recombinant Mouse Chemerin (aa 17-156) Protein

Carrier Free

Catalog # Availability Size / Price Qty
2325-CM-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2325-CM-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (14)
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Reviews (2)

Recombinant Mouse Chemerin (aa 17-156) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human ChemR23. The ED50 for this effect is 6-30 ng/mL.
Source
E. coli-derived mouse Chemerin protein
Thr17-Ser156
Accession #
N-terminal Sequence
Analysis
Thr17
Predicted Molecular Mass
16 kDa
SDS-PAGE
18 kDa, reducing conditions

Product Datasheets

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2325-CM (with carrier)

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2325-CM/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2325-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2325-CM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Chemerin

Mouse Chemerin, also known as Tazarotene-induced Gene-2 (TIG2), is a new, but distant member of the cystatin superfamily (1 - 3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (aa) (2, 3). Chemerin is synthesized as a 162 aa precursor that contains a hydrophobic N-terminal sequence, an intervening 140 aa cystatin-fold containing domain, and a six aa C-terminal prosegment (4 - 6). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the characteristic fold (4, 7). The precursor molecule is described as undergoing proteolytic processing at both termini by unknown proteases. The N-terminal 16 residue hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (5, 8). In either case it gives rise to a soluble proform that undergoes further processing at the C-terminus (5). In mouse, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 17 - 156) (5 - 7). A shorter form has been described in human (7). The activity seems to be concentrated in the nine aa’s preceding the prosegment (aa 148 - 156). Retention of the prosegment blocks activity (4). The 140 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (5). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature mouse Chemerin shares 67%, 84% and 82% aa sequence identity to human, rat and hamster Chemerin, respectively (6). There is apparently cross-species activity for the protein (6).

References
  1. Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91.
  2. Storici, P. et al. (1996) Eur. J. Biochem. 238:769.
  3. Zanetti, M. (2004) J. Leukoc. Biol. 75:39.
  4. Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956.
  5. Wittamer, V. et al. (2003) J. Exp. Med. 198:977.
  6. Busmann, A. et al. (2004) J. Chromatog. B 811:217.
  7. Meder, W. et al. (2003) FEBS Lett. 555:495.
  8. Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.
Long Name
Retinoic Acid Receptor Responder Protein 2
Entrez Gene IDs
5919 (Human); 71660 (Mouse)
Alternate Names
Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433

Citations for Recombinant Mouse Chemerin (aa 17-156) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Expression of CCRL2 Inhibits Tumor Growth by Concentrating Chemerin and Inhibiting Neoangiogenesis
    Authors: D Al Delbany, V Robert, I Dubois-Ved, A Del Prete, M Vernimmen, A Radi, A Lefort, F Libert, V Wittamer, S Sozzani, M Parmentier
    Cancers, 2021-10-05;13(19):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. The antitumoral effects of chemerin are independent from leukocyte recruitment and mediated by inhibition of neoangiogenesis
    Authors: I Dubois-Ved, D Al Delbany, O De Henau, V Robert, M Vernimmen, F Langa, A Lefort, F Libert, V Wittamer, M Parmentier
    Oncotarget, 2021-09-14;12(19):1903-1919.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Loss of chemerin triggers bone remodeling in vivo and in vitro
    Authors: L Han, Y Zhang, S Wan, Q Wei, W Shang, G Huang, P Fang, W Min
    Molecular Metabolism, 2021-08-17;0(0):101322.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Discovery of chemerin as the new chemoattractant of human mesenchymal stem cells
    Authors: I Kim, H Park, I Hwang, D Moon, H Yun, EJ Lee, HS Kim
    Cell & bioscience, 2021-07-01;11(1):120.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Chemerin-induced macrophages pyroptosis in fetal brain tissue leads to cognitive disorder in offspring of diabetic dams
    Authors: Z Liang, L Han, D Sun, Y Chen, Q Wu, L Zhang, M Zhou, D Chen
    J Neuroinflammation, 2019-11-16;16(1):226.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Adipokine Chemerin Bridges Metabolic Dyslipidemia and Alveolar Bone Loss in Mice
    Authors: Erivan S Ramos-Juni
    J. Bone Miner. Res, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Nat Commun, 2016-08-19;7(0):12528.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. The role of GPR1 signaling in mice corpus luteum
    Authors: Jian V Zhang
    J. Endocrinol., 2016-05-05;230(1):55-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease.
    Authors: Graham K, Zhang J, Lewen S, Burke T, Dang T, Zoudilova M, Sobel R, Butcher E, Zabel B
    PLoS ONE, 2014-12-01;9(12):e112925.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Endothelial cell-derived chemerin promotes dendritic cell transmigration.
    Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
    J Immunol, 2014-01-27;192(5):2366-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses.
    J. Exp. Med., 2012-07-02;209(8):1427-35.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  12. Chemerin peptides promote phagocytosis in a ChemR23- and Syk-dependent manner.
    Authors: Cash JL, Christian AR, Greaves DR
    J. Immunol., 2010-04-02;184(9):5315-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  13. Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
    Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
    J. Immunol., 2009-10-19;183(10):6489-99.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  14. Synthetic chemerin-derived peptides suppress inflammation through ChemR23.
    Authors: Cash JL, Hart R, Russ A, Dixon JP, Colledge WH, Doran J, Hendrick AG, Carlton MB, Greaves DR
    J. Exp. Med., 2008-04-07;205(4):767-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse Chemerin (aa 17-156) Protein

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Recombinant Mouse Chemerin (aa 17-156) Protein
By Diego Rodriguez on 01/06/2017
Application: Apoptosis assay
Reason for Rating: The product performed as we espected.

Recombinant Mouse Chemerin (aa 17-156) Protein
By Diego Rodríguez-Penas on 06/23/2016
Application: Apoptosis assay