Recombinant Mouse Chondroadherin His-tag Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Mouse Chondroadherin His-tag Protein, CF Bioactivity
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Product Details

Recombinant Mouse Chondroadherin His-tag Protein, CF Summary

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.08-048 μg/mL
Mouse myeloma cell line, NS0-derived mouse Chondroadherin protein
Ala21-Thr349, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained. Protein identity confirmed via Mass Spectrometry.
Predicted Molecular Mass
38 kDa
33-38 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse Chondroadherin (Catalog # 10033-CH)induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is0.08-0.48 μg/mL.


2 μg/lane of Recombinant Mouse Chondroadherin was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 33-38 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Chondroadherin

Chondroadherin (CHAD), also known as SLRR4A, is a 38 kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the extracellular matrix (ECM) (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature mouse CHAD is a 338 amino acid (aa)  protein that contains twelve leucine-rich repeats (LRRs) including ten tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions. CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD also interacts with both N- and C-terminal globular domains of type VI collagen (10). Mouse CHAD shares 95% and 98% aa sequence identity with human and rat CHAD, respectively.

  1. Neame, P.J. et al. (1994) J. Biol. Chem. 269:21547.
  2. Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
  3. Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
  4. Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
  5. Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
  6. Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
  7. Batista, M. et al. (2014) Matrix Biol. 38:84.
  8. Camper, L. et al. (1997) J. Cell Biol. 138:1159.
  9. Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
  10. Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.
Entrez Gene IDs
1101 (Human); 12643 (Mouse); 29195 (Rat)
Alternate Names
CHAD; chondroadherin proteoglycan; Chondroadherin; SLRR4A; SLRR4ACartilage leucine-rich protein


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