Recombinant Mouse CLEC-2/CLEC1B Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 0.5 µg/mL (100 μL/well), Recombinant Mouse
    CLEC-2/CLEC1B binds with a typical ED50 of
    0.1-0.5 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Met53-Leu229, with an N-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    His
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    24-39 kDa, reducing conditions
9117-CL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Mouse Podoplanin Fc Chimera (Catalog #
3244-PL) is coated at 0.5 µg/mL, Recombinant Mouse
CLEC-2/
CLEC1B (Catalog #
9117-CL) binds with a typical ED50 of
0.1-0.5 μg/mL.
Background: CLEC-2/CLEC1B
C-type lectin-like receptor 2, also known as CLEC-1B, is a 32 kDa type II transmembrane glycoprotein that is expressed on platelets, megakaryocyte, and liver sinusoidal cells (1-4). Mature mouse CLEC-2 consists of a 31 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) with one C-type lectin domain. Within the ECD, mouse CLEC-2 shares 59% and 90% aa sequence identity with human and rat CLEC-2, respectively. Alternative splicing generates a short isoform that lacks the transmembrane segment. CLEC-2 binding to Podoplanin trigger platelet activation, shedding of GPVI, and cleavage of Fcg RIIA/CD32a (5-8). CLEC-2 expression on platelets is required for the development of lymphatic vessels and valves as well as the maintenance of lymph node high endothelial veinules (HEV) during lymphocyte crossing (7-10). CLEC-2 additionally binds to fucoidan, HIV-1, and the platelet-aggregating snake venom protein Rhodocytin/Aggretin (2, 3, 11). In the liver, soluble CLEC-2 promotes M2 macrophage differentiation and limits inflammatory cytokine production (12).
  • References:
    1. Lee, R.H. and W. Bergmeier (2016) J. Thromb. Haemost. Epub PMID 26749528.
    2. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
    3. Chaipan, C. et al. (2006) J. Virol. 80:8951.
    4. Gitz, E. et al. (2014) Blood 124:2262.
    5. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
    6. Christou, C.M. et al. (2008) Biochem. J. 411:133.
    7. Herzog, B.H. et al. (2013) Nature 502:105.
    8. Sweet, D.T. et al. (2015) J. Clin. Invest. 125:2995.
    9. Hess, P.R. et al. (2014) J. Clin. Invest. 124:273.
    10. Osada, M. et al. (2012) J. Biol. Chem. 287:22241.
    11. Manne, B.K. et al. (2013) J. Biol. Chem. 288:7717.
    12. Wu, X. et al. (2015) EbioMedicine 2:214.
  • Long Name:
    C-type Lectin-like Receptor 2
  • Entrez Gene IDs:
    51266 (Human); 56760 (Mouse)
  • Alternate Names:
    1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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