>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When
Mouse Podoplanin Fc Chimera (Catalog # 3244-PL)
is coated at 0.5 µg/mL (100 μL/well), Recombinant Mouse CLEC-2/CLEC1B binds with a typical ED50 of 0.1-0.5 μg/mL.
Mouse myeloma cell line, NS0-derived Met53-Leu229, with an N-terminal 6-His tag
When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 0.5 µg/mL, Recombinant Mouse CLEC-2/ CLEC1B (Catalog # 9117-CL) binds with a typical ED50 of 0.1-0.5 μg/mL.
C-type lectin-like receptor 2, also known as CLEC-1B, is a 32 kDa type II transmembrane glycoprotein that is expressed on platelets, megakaryocyte,
and liver sinusoidal cells (1-4). Mature mouse CLEC-2 consists of a 31 amino
acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa
extracellular domain (ECD) with one C-type lectin domain. Within the ECD, mouse
CLEC-2 shares 59% and 90% aa sequence identity with human and rat CLEC-2,
respectively. Alternative splicing generates a short isoform that lacks the
transmembrane segment. CLEC-2 binding to Podoplanin trigger platelet
activation, shedding of GPVI, and cleavage of Fcg RIIA/CD32a (5-8). CLEC-2 expression on
platelets is required for the development of lymphatic vessels and valves as
well as the maintenance of lymph node high endothelial veinules (HEV) during
lymphocyte crossing (7-10). CLEC-2 additionally binds to fucoidan, HIV-1, and the
platelet-aggregating snake venom protein Rhodocytin/Aggretin (2, 3, 11). In the
liver, soluble CLEC-2 promotes M2 macrophage differentiation and limits inflammatory
cytokine production (12).
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Christou, C.M. et al. (2008) Biochem. J. 411:133.
Herzog, B.H. et al. (2013) Nature 502:105.
Sweet, D.T. et al. (2015) J. Clin. Invest. 125:2995.
Hess, P.R. et al. (2014) J. Clin. Invest. 124:273.
Osada, M. et al. (2012) J. Biol. Chem. 287:22241.
Manne, B.K. et al. (2013) J. Biol. Chem. 288:7717.
Wu, X. et al. (2015) EbioMedicine 2:214.
C-type Lectin-like Receptor 2
Entrez Gene IDs:
51266 (Human); 56760 (Mouse)
1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721
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