Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF Summary
Accession # Q67DU8
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse CLEC4B2 Fc Chimera (Catalog # 10087-DC) binds fluorescein-conjugated S. aureus bioparticles. The ED50 for this effect is 0.25-1.25 μg/mL.
CLEC4B2 (C-type Lectin domain family 4, member b2), also called dendritic cell immunoactivating receptor 1 (DCAR1) and antigen presenting cell lectin-like receptor A1 (APLRA1), is a type II membrane protein belonging to the cell surface C-type lectin domain family (1, 2). Mouse CLEC4B2 is synthesized as a 208 amino acid (aa) precursor that contains a 22 aa transmembrane segment and a 167 aa extracellular domain (ECD) containing a C-type lectin domain. The ECD of mouse CLEC4B2 shares 71% and 50% aa identity with rat CLEC4B2 ECD and human CLEC4B2 ECD, respectively. Activation of CLEC4B2 in the epidermis regulates immune defenses. CLEC4B2 potentially has a second role in host defense. CLEC4B2 is specifically required for the innate immune response to fungal infection and wounding and place this GPCR further upstream than any other known component of innate immune recognition and signaling in C. elegans epidermis.
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