Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10087-DC-050
Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF Bioactivity
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Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED50 for this effect is 0.25-1.25 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CLEC4B2/mDCAR1 protein
MDPMouse IgG2A
(Glu98-Lys330)
IEGR Mouse CLEC4B2
(Gln42-Leu208)
Accession # Q67DU8
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47 kDa
SDS-PAGE
50-59 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10087-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

Recombinant Mouse CLEC4B2 Fc Chimera (Catalog # 10087-DC) binds fluorescein-conjugated S. aureus bioparticles. The ED50 for this effect is 0.25-1.25 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC4B2/mDCAR1

CLEC4B2 (C-type Lectin domain family 4, member b2), also called dendritic cell immunoactivating receptor 1 (DCAR1) and antigen presenting cell lectin-like receptor A1 (APLRA1), is a type II membrane protein belonging to the cell surface C-type lectin domain family (1, 2). Mouse CLEC4B2 is synthesized as a 208 amino acid (aa) precursor that contains a 22 aa transmembrane segment and a 167 aa extracellular domain (ECD) containing a C-type lectin domain. The ECD of mouse CLEC4B2 shares 71% and 50% aa identity with rat CLEC4B2 ECD and human CLEC4B2 ECD, respectively. Activation of CLEC4B2 in the epidermis regulates immune defenses. CLEC4B2 potentially has a second role in host defense. CLEC4B2 is specifically required for the innate immune response to fungal infection and wounding and place this GPCR further upstream than any other known component of innate immune recognition and signaling in C. elegans epidermis.

References
  1. Flornes, L. M. et al. (2004) Immunogenetics 56:506.
  2. Kaden, S. A. et al. (2009) J. Immunol. 183:5069.
  3. Zugasti, O. et al. (2014) Nat. Immunol. 15:833.
Long Name
C-type lectin domain family 4, Member b2
Entrez Gene IDs
381809 (Mouse)
Alternate Names
CLEC4B2; mDCAR1

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