Recombinant Mouse CLEC4F/CLECSF13 Protein, CF

R&D Systems | Catalog # 2784-CL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CLEC4F/CLECSF13 Protein (2784-CL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P70194

Applications

Binding Activity
View all CLEC4F/CLECSF13 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CLEC4F/CLECSF13 protein
Ala65-Gly548, with an N-terminal 9-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

55 kDa

SDS-PAGE

65-85 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles.
The ED50 for this effect is 0.75-3.75 µg/mL.

Formulation, Preparation, and Storage

2784-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1 - 3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N-acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
  3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
  4. Accession # P70194.
  5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
  6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
  7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
  8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
  9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
  10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
  11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Long Name

C-type Lectin Domain Family 4, Member F

Alternate Names

CLECSF13, Galactose Particle Receptor, KCLR, KCR

Entrez Gene IDs

165530 (Human); 51811 (Mouse); 114598 (Rat)

Gene Symbol

CLEC4F

UniProt

P70194

Additional CLEC4F/CLECSF13 Products

Product Documents for Recombinant Mouse CLEC4F/CLECSF13 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CLEC4F/CLECSF13 Protein, CF

For research use only

Related Research Areas

C-type Lectin Family

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