Recombinant Mouse Complement Component C3a Protein, CF

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Recombinant Mouse Complement Component C3a Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance IL-6 secretion by human peripheral blood mononuclear cells (PBMC). Fischer, W.H. et al. (1999) J. Immunol. 162:453. The ED50 for this effect is typically 0.8-4 µg/mL
E. coli-derived mouse Complement Component C3a protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
9.2 kDa
9 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Complement Component C3a

C3a is an anaphylotoxin polypeptide comprising amino acids (aa) 671-748 of the Complement C3 precursor protein (1-4). Anaphylatoxins are proteolytically generated from the C3, C4 and C5 alpha chains by convertases formed by other complement fragments (2). They share 30-36% aa identity, and mediate inflammatory responses that vary in strength in the order C5a > C3a > C4a (2). Like C4a and C5a, the 78 aa, 9 kDa mouse C3a contains six conserved cysteine residues that form a knot structure and possess an overall basic charge (4, 5). It is not glycosylated (4). The C-terminal regions of C3a and C4a show antimicrobial activity, while C5a is chemotactic but not antimicrobial (5). Mouse C3a shares 94% aa sequence identity with rat, and 65-69% with human, guinea pig, bovine, porcine and canine C3a. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative (1, 2). It binds the G-protein coupled C3a receptor (C3aR) on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells (6, 7). C3a contributes to both innate and adaptive immunity. It activates mast cells and neutrophils, triggering robust mast cell degranulation in airways during asthmatic allergen challenges (8). It enhances lipopolysaccharide-induced prostaglandin, cytokine and chemokine secretion by macrophages and other cells (1, 6, 9, 10). It assists in Th2-type inflammatory reactions, promotes Th1 cell maturation, and down-regulates regulatory T cell differentiation (8, 11). It stimulates leukocyte chemotaxis and smooth muscle contraction (8, 9).  Endogenous carboxypeptidase-N can remove the arginine at the C-terminus of the anaphylatoxins to create desArg forms (1). C3adesArg, also called ASP (Acylation-Stimulating Protein) is an adipocyte-derived protein that binds the C5L2 receptor (GPR77) and stimulates adipose tissue triglyceride synthesis (2, 7, 12). The anaphylactic activity of ASP is weaker than that of C3a (7, 10). C5L2 is also involved in C3a and C5a activity (12).

  1. Markiewski, M. and J.D. Lambris (2007) Am. J. Pathol. 171:715.
  2. Haas, P-J. and J. van Strijp (2007) Immunol. Res. 37:161.
  3. Domdey, H. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7619.
  4. Hugli, T.E. (1975) J. Biol. Chem. 250:8293.
  5. Pasupuleti, M. et al. (2007) J. Biol. Chem. 282:2520.
  6. Thurman, J.M. et al. (2007) J. Immunol. 178:1819.
  7. Kalant, D. et al. (2005) J. Biol. Chem. 280:23936.
  8. Ali, H. and R.A. Panettieri (2005) Respir. Res. 6:19.
  9. Honczarenko, M. et al. (2005) J. Immunol. 175:3698.
  10. Fischer, W.H. et al. (1999) J. Immunol. 162:453.
  11. van der Touw, W. et al. (2013) J. Immunol. 190:5921.
  12. Chen, N-J. et al. (2007) Nature 446:203.
Entrez Gene IDs
718 (Human); 12266 (Mouse)
Alternate Names
Anaphylatoxin; C3; Complement Component C3a

Citations for Recombinant Mouse Complement Component C3a Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-alpha
    Authors: B Gong, D Guo, C Zheng, Z Ma, J Zhang, Y Qu, X Li, G Li, L Zhang, Y Wang
    Journal of Neuroinflammation, 2022;19(1):159.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells
    Authors: M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu
    Journal of Neuroinflammation, 2022;19(1):182.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The complement C3-complement factor D-C3a receptor signalling axis regulates cardiac remodelling in right ventricular failure
    Authors: S Ito, H Hashimoto, H Yamakawa, D Kusumoto, Y Akiba, T Nakamura, M Momoi, J Komuro, T Katsuki, M Kimura, Y Kishino, S Kashimura, A Kunitomi, M Lachmann, M Shimojima, G Yozu, C Motoda, T Seki, T Yamamoto, Y Shinya, T Hiraide, M Kataoka, T Kawakami, K Suzuki, K Ito, H Yada, M Abe, M Osaka, H Tsuru, M Yoshida, K Sakimura, Y Fukumoto, M Yuzaki, K Fukuda, S Yuasa
    Nature Communications, 2022;13(1):5409.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages
    Authors: A Cohen, EE Jeng, M Voorhies, J Symington, N Ali, RA Rodriguez, MC Bassik, A Sil
    PloS Pathogens, 2022;18(9):e1010237.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Heme catabolism by tumor-associated macrophages controls metastasis formation
    Authors: FM Consonni, A Bleve, MG Totaro, M Storto, P Kunderfran, A Termanini, F Pasqualini, C Alì, C Pandolfo, F Sgambellur, G Grazia, M Santinami, A Maurichi, M Milione, M Erreni, A Doni, M Fabbri, L Gribaldo, E Rulli, MP Soares, V Torri, R Mortarini, A Anichini, A Sica
    Nature Immunology, 2021;22(5):595-606.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3
    Authors: Z Zheng, YN Li, S Jia, M Zhu, L Cao, M Tao, J Jiang, S Zhan, Y Chen, PJ Gao, W Hu, Y Wang, C Shao, Y Shi
    Nature Communications, 2021;12(1):6202.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. C3a elicits unique migratory responses in immature low-density neutrophils
    Authors: BE Hsu, J Roy, J Mouhanna, RF Rayes, L Ramsay, S Tabariès, MG Annis, IR Watson, JD Spicer, S Costantino, PM Siegel
    Oncogene, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Complement activation contributes to perioperative neurocognitive disorders in mice
    Authors: C Xiong, J Liu, D Lin, J Zhang, N Terrando, A Wu
    J Neuroinflammation, 2018;15(1):254.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Early structural changes of the heart after experimental polytrauma and hemorrhagic shock
    Authors: CK Braun, M Kalbitz, R Halbgebaue, P Eisele, DAC Messerer, S Weckbach, A Schultze, S Braumüller, F Gebhard, MS Huber-Lang
    PLoS ONE, 2017;12(10):e0187327.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA (Standard)
  10. Tissue plasminogen activator mediates deleterious complement cascade activation in stroke
    Authors: XJ Zhao, TM Larkin, MA Lauver, S Ahmad, AF Ducruet
    PLoS ONE, 2017;12(7):e0180822.
    Applications: Bioassay
  11. IgE Trimers Drive SPE-7 Cytokinergic Activity
    Authors: HJ Bax, H Bowen, RL Beavil, R Chung, M Ward, AM Davies, TS Dodev, JM McDonnell, AJ Beavil, BJ Sutton, HJ Gould
    Sci Rep, 2017;7(1):8164.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Complement Component 3 Regulates IFN-? Production by Plasmacytoid Dendritic Cells following TLR7 Activation by a Plant Virus-like Nanoparticle
    Authors: Alain Lamarre
    J. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo


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Recombinant Mouse Complement Component C3a Protein, CF
By Anonymous on 05/09/2018
Application: Immunoassay Standard