Recombinant Mouse Complement Component C5a Protein 2150-C5-025: R&D Systems

Recombinant Mouse Complement Component C5a Protein

(23 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Klos, A. et al. (1992) Biochemistry 31:11274. The ED50 for this effect is 5-20 ng/mL.
  • Source
    E. coli-derived Asn679-Arg755, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    9.0 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2150-C5
 
2150-C5/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is 5-20 ng/mL.

1 μg/lane of Recombinant Mouse Complement Component C5a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.

Background: Complement Component C5a

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

  • References:
    1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
    2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
    3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
    4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
    5. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  • Entrez Gene IDs:
    727 (Human); 15139 (Mouse)
  • Alternate Names:
    C5; C5A; Complement Component C5a; HE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. The Ribosomal Protein S19 Suppresses Antitumor Immune Responses via the Complement C5a Receptor 1
    Authors: MM Markiewski, SK Vadrevu, SK Sharma, NK Chintala, S Ghouse, JH Cho, DP Fairlie, Y Paterson, A Astrinidis, M Karbownicz
    J. Immunol., 2017;198(7):2989-2999.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing
    Authors: S Bergdolt, A Kovtun, Y Hägele, A Liedert, T Schinke, M Amling, M Huber-Lang, A Ignatius
    PLoS ONE, 2017;12(6):e0179512.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg
    Authors: Y Wang, P Lai, X Chen, C He, X Huang, S Geng, C Luo, S Wu, W Ling, L Zhong, Z Lu, P Li, J Weng, X Du
    Sci Rep, 2017;7(1):3603.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Sexual dimorphism of liver metastasis by murine pancreatic neuroendocrine tumors is affected by expression of complement C5
    Oncotarget, 2016;7(21):30585-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1
    J Neurosci, 2016;36(18):5055-70.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  6. Blocking neutrophil diapedesis prevents hemorrhage during thrombocytopenia.
    Authors: Hillgruber C, Poppelmann B, Weishaupt C, Steingraber A, Wessel F, Berdel W, Gessner J, Ho-Tin-Noe B, Vestweber D, Goerge T
    J Exp Med, 2015;212(8):1255-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Distinct cellular sources of hepoxilin A3 and leukotriene B4 are used to coordinate bacterial-induced neutrophil transepithelial migration.
    Authors: Pazos M, Pirzai W, Yonker L, Morisseau C, Gronert K, Hurley B
    J Immunol, 2015;194(3):1304-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Cutting edge: critical role for C5aRs in the development of septic lymphopenia in mice.
    Authors: Grailer J, Fattahi F, Dick R, Zetoune F, Ward P
    J Immunol, 2015;194(3):868-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer.
    Authors: Yatime, Laure, Maasch, Christia, Hoehlig, Kai, Klussmann, Sven, Andersen, Gregers, Vater, Axel
    Nat Commun, 2015;6(0):6481.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Complement 5a Enhances Hepatic Metastases of Colon Cancer via Monocyte Chemoattractant Protein-1-mediated Inflammatory Cell Infiltration.
    Authors: Piao C, Cai L, Qiu S, Jia L, Song W, Du J
    J Biol Chem, 2015;290(17):10667-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara.
    Authors: Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M
    J Immunol, 2015;194(3):1164-8.
    Species: Mouse
    Sample Type: Protein
    Application: WB Ctrl
  12. High expression of C5L2 correlates with high proinflammatory cytokine expression in advanced human atherosclerotic plaques.
    Authors: Vijayan S, Asare Y, Grommes J, Soehnlein O, Lutgens E, Shagdarsuren G, Togtokh A, Jacobs M, Fischer J, Bernhagen J, Weber C, Schober A, Shagdarsuren E
    Am J Pathol, 2014;184(7):2123-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. IFN-gamma-producing NKT cells exacerbate sepsis by enhancing C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils.
    Authors: Kim J, Oh S, Ahn S, Chung D
    Eur J Immunol, 2014;44(7):2025-35.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  14. C5L2 is required for C5a-triggered receptor internalization and ERK signaling.
    Authors: Hsu W, Yang F, Lin C, Hsieh S, Chen N
    Cell Signal, 2014;26(7):1409-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis.
    Authors: Hoehlig, Kai, Maasch, Christia, Shushakova, Nelli, Buchner, Klaus, Huber-Lang, Markus, Purschke, Werner G, Vater, Axel, Klussmann, Sven
    Mol Ther, 2013;21(12):2236-46.
    Species: N/A
    Sample Type: Protein
    Application: Surface Plamon Resonance
  16. The interaction between C5a and both C5aR and C5L2 receptors is required for production of G-CSF during acute inflammation.
    Authors: Bosmann M, Haggadone M, Zetoune F, Sarma J, Ward P
    Eur J Immunol, 2013;43(7):1907-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury.
    Authors: Bosmann, Markus, Grailer, Jamison, Ruemmler, Robert, Russkamp, Norman F, Zetoune, Firas S, Sarma, J Vidya, Standiford, Theodore, Ward, Peter A
    FASEB J, 2013;27(12):5010-21.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  18. Local complement-targeted intervention in periodontitis: proof-of-concept using a C5a receptor (CD88) antagonist.
    Authors: Abe T, Hosur K, Hajishengallis E, Reis E, Ricklin D, Lambris J, Hajishengallis G
    J Immunol, 2012;189(11):5442-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: Bioassay
  19. Opposing roles for complement component C5a in tumor progression and the tumor microenvironment.
    Authors: Gunn L, Ding C, Liu M, Ma Y, Qi C, Cai Y, Hu X, Aggarwal D, Zhang H, Yan J
    J Immunol, 2012;189(6):2985-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. The C5a Receptor on Mast Cells Is Critical for the Autoimmune Skin-blistering Disease Bullous Pemphigoid.
    Authors: Heimbach L, Li Z, Berkowitz P
    J. Biol. Chem., 2011;286(17):15003-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
    Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
    J. Immunol., 2011;186(2):869-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a.
    Authors: Chen NJ, Mirtsos C, Suh D, Lu YC, Lin WJ, McKerlie C, Lee T, Baribault H, Tian H, Yeh WC
    Nature, 2007;446(7132):203-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  23. Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice.
    Authors: Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, Baumruker T, Billich A
    Immunol. Lett., 2007;109(1):56-63.
Expand to show all 23 Citations

Have you used Recombinant Mouse Complement Component C5a Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information