Recombinant Mouse Contactin-1 Protein, CF

Catalog # Availability Size / Price Qty
9665-CN-100
Recombinant Mouse Contactin-1 Protein Bioactivity
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse Contactin-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Contactin‑1 is coated at 1 µg/mL, 100 μL/well, Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR) binds with an ED50 of 0.03-0.18 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Contactin-1 protein
Mouse Contactin-1
(Asp21-Thr999)
Accession # AAH66864
HPGGGSGGGSGGGS HHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp21
Predicted Molecular Mass
111 kDa
SDS-PAGE
119-135 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9665-CN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Contactin-1 Protein Bioactivity View Larger

When Recombinant Mouse Contactin-1 (Catalog # 9665-CN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR) binds with an ED50 of 0.03-0.18 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Contactin-1

Contactin-1 (CNTN1), also known as F3 and F11, is an approximately 135 kDa GPI-anchored member of the Contactin family of neuronal adhesion glycoproteins (1). Mature mouse Contactin-1 contains 6 Ig-like domains and 4 fibronectin type III-like domains, and it shares 96% and 100% amino acid (aa) sequence identity with human and rat Contactin-1, respectively (2, 3). Alternative splicing generates an isoform with an 11 aa deletion N-terminal to the first Ig-like domain and another isoform that is substituted and truncated following the sixth Ig-like domain. In humans, Contactin-1 is critical in the development of the central nervous system, and a soluble form of Contactin-1 is released into the cerebrospinal fluid (4). Contactin-1 is expressed on neurons and their precursors at sites of glial cell contact with neuronal processes (5-7). Within paranodal regions of the axon, Contactin-1 associates in cis with the transmembrane protein Caspr (7). It binds in trans to other neuronal adhesion proteins (e.g. NrCAM, Neurofascin, Tenascin R), the phosphatase PTPRZ, and the chondroitin sulfate CS-E (8-13). It also binds and activates both Notch-1 and Notch-2 (14). In cancer, Contactin-1 is up-regulated by VEGF-C and is required for VEGF R3/Flt-4 induced tumor cell invasion and metastasis (15). In humans, mutations in CNTN1 cause a familial form of lethal congenital myopathy (16).

References
  1.  Shimoda, Y. and K. Watanabe (2009) Cell Adh. Migr. 3:64.
  2. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
  3. Berglund, E.O. and B. Ranscht (1994) Genomics 21:571.
  4. Durbec, P. et al. (1992) J. Cell Biol. 117:877.
  5. Gennarini, G. et al. (1990) Acta Histochem. 38:65.
  6. Bizzoca, A. et al. (2012) Dev. Biol. 365:133.
  7. Boyle, M.E.T. et al. (2001) Neuron 30:385.
  8. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  9. Xenaki, D. et al. (2011) Development 138:519.
  10. Volkmer, H. et al. (1998) J. Cell Biol. 142:1083.
  11. Pesheva, P. et al. (1993) Neuron 10:69.
  12. Peles, E. et al. (1995) Cell 82:251.
  13. Mikami, T. et al. (2009) J. Biol. Chem. 284:4494.
  14. Hu, Q.-D. et al. (2003) Cell 115:163.
  15. Su, J.-L. et al. (2006) Cancer Cell 9:209.
  16. Compton AG (2008) Am J Hum Genet 83:714.
Entrez Gene IDs
1272 (Human); 12805 (Mouse)
Alternate Names
CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Contactin-1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Contactin-1 Protein, CF and earn rewards!

Have you used Recombinant Mouse Contactin-1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review