Recombinant Mouse Contactin-1 Protein, CF

R&D Systems | Catalog # 9665-CN

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Contactin-1 Protein (9665-CN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAH66864

Applications

Bioactivity
View all Contactin-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Contactin-1 protein
Mouse Contactin-1
(Asp21-Thr999)
Accession # AAH66864		 HPGGGSGGGSGGGS  HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp21

Predicted Molecular Mass

111 kDa

SDS-PAGE

119-135 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Contactin‑1 is coated at 1 µg/mL, 100 μL/well, Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR) binds with an ED50 of 0.03-0.18 μg/mL.

Scientific Data Images for Recombinant Mouse Contactin-1 Protein, CF

Recombinant Mouse Contactin-1 Protein Bioactivity

Recombinant Mouse Contactin-1 Protein Bioactivity

When Recombinant Mouse Contactin-1 (Catalog # 9665-CN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR) binds with an ED50 of 0.03-0.18 µg/mL.

Formulation, Preparation, and Storage

9665-CN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-1

Contactin-1 (CNTN1), also known as F3 and F11, is an approximately 135 kDa GPI-anchored member of the Contactin family of neuronal adhesion glycoproteins (1). Mature mouse Contactin-1 contains 6 Ig-like domains and 4 fibronectin type III-like domains, and it shares 96% and 100% amino acid (aa) sequence identity with human and rat Contactin-1, respectively (2, 3). Alternative splicing generates an isoform with an 11 aa deletion N-terminal to the first Ig-like domain and another isoform that is substituted and truncated following the sixth Ig-like domain. In humans, Contactin-1 is critical in the development of the central nervous system, and a soluble form of Contactin-1 is released into the cerebrospinal fluid (4). Contactin-1 is expressed on neurons and their precursors at sites of glial cell contact with neuronal processes (5-7). Within paranodal regions of the axon, Contactin-1 associates in cis with the transmembrane protein Caspr (7). It binds in trans to other neuronal adhesion proteins (e.g. NrCAM, Neurofascin, Tenascin R), the phosphatase PTPRZ, and the chondroitin sulfate CS-E (8-13). It also binds and activates both Notch-1 and Notch-2 (14). In cancer, Contactin-1 is up-regulated by VEGF-C and is required for VEGF R3/Flt-4 induced tumor cell invasion and metastasis (15). In humans, mutations in CNTN1 cause a familial form of lethal congenital myopathy (16).

References

  1.  Shimoda, Y. and K. Watanabe (2009) Cell Adh. Migr. 3:64.
  2. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
  3. Berglund, E.O. and B. Ranscht (1994) Genomics 21:571.
  4. Durbec, P. et al. (1992) J. Cell Biol. 117:877.
  5. Gennarini, G. et al. (1990) Acta Histochem. 38:65.
  6. Bizzoca, A. et al. (2012) Dev. Biol. 365:133.
  7. Boyle, M.E.T. et al. (2001) Neuron 30:385.
  8. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  9. Xenaki, D. et al. (2011) Development 138:519.
  10. Volkmer, H. et al. (1998) J. Cell Biol. 142:1083.
  11. Pesheva, P. et al. (1993) Neuron 10:69.
  12. Peles, E. et al. (1995) Cell 82:251.
  13. Mikami, T. et al. (2009) J. Biol. Chem. 284:4494.
  14. Hu, Q.-D. et al. (2003) Cell 115:163.
  15. Su, J.-L. et al. (2006) Cancer Cell 9:209.
  16. Compton AG (2008) Am J Hum Genet 83:714.

Alternate Names

CNTN1, Contactin1, F11, F3cam

Entrez Gene IDs

1272 (Human); 12805 (Mouse)

Gene Symbol

CNTN1

UniProt

AAH66864

Additional Contactin-1 Products

Product Documents for Recombinant Mouse Contactin-1 Protein, CF

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Product Specific Notices for Recombinant Mouse Contactin-1 Protein, CF

For research use only

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail