Recombinant Mouse Contactin-6 Protein, CF

R&D Systems | Catalog # 5890-CN

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Contactin-6 Protein (5890-CN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9JMB8

Applications

Bioactivity
View all Contactin-6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-6 protein
Glu20-Ser998, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu20

Predicted Molecular Mass

109.2 kDa

SDS-PAGE

140-160 kDa, reducing conditions

Activity

Bioassay data are not available.


Formulation, Preparation, and Storage

5890-CN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Contactin-6

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3 - 5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5 - 7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

References

  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
  3. Lee, S. et al. (2000) Gene 245:253.
  4. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
  5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
  6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
  7. Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
  8. Ye, H. et al. (2008) EMBO J. 27:188.

Alternate Names

CNTN6, Contactin6, NB3

Entrez Gene IDs

27255 (Human); 53870 (Mouse); 27256 (Rat)

Gene Symbol

CNTN6

UniProt

Q9JMB8 (Mouse)

Additional Contactin-6 Products

Product Documents for Recombinant Mouse Contactin-6 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Contactin-6 Protein, CF

For research use only

Related Research Areas

Notch Pathway

Citations for Recombinant Mouse Contactin-6 Protein, CF

Customer Reviews for Recombinant Mouse Contactin-6 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Contactin-6 Protein, CF and earn rewards!

Have you used Recombinant Mouse Contactin-6 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes