Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

R&D Systems | Catalog # 8479-DT

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse DcTRAILR1/TNFRSF23 Protein (8479-DT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9ER63

Applications

Bioactivity
View all DcTRAILR1/TNFRSF23 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DcTRAIL R1/TNFRSF23 protein
Met30-Ser156, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met30

Predicted Molecular Mass

15 kDa

SDS-PAGE

23-30 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse DcTRAIL R1/TNFSF23 is immobilized at 0.1 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% optimal binding response is approximately 2-12 ng/mL.

Formulation, Preparation, and Storage

8479-DT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DcTRAILR1/TNFRSF23

Decoy TRAIL receptor 1 (DcTRAIL R1), also called TNFRSF23 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein that binds TRAIL/TNFSF10 with high affinity (1). Mouse DcTRAIL R1 consists of a 29 amino acid (aa) signal peptide, a 126 aa mature region and a 21 aa GPI anchor signal. The mature region shows a TNFR cysteine rich domain (CRD) with three tandem A1/B2 repeats (2). Compared to signaling TRAIL receptors, DcTRAIL R1 does not trigger death signaling due to its lack of an intracellular death domain. Also, unlike many TNF receptors family members, DcTRAIL R1 does not contain a TRAFF-binding motif. Thus DcTRAIL R1 failed to induce NF kappa B activity. Structural studies revealed a strict species selectivity of the mouse DcTRAIL R1 for mouse and not human TRAIL based on amino acid specific electrostatic interactions between the two proteins (2). Mouse DcTRAIL R1, DcTRAIL R2 (TNFRSF22), and TNFRSF26 are encoded by clustered genes within the Kcnq1 domain of chromosome 7. The human counterpart of the Kcnq1 domain does not encode a functional homolog of the murine DcTRAIL R1 (3). Mouse DcTRAIL R1 is weakly expressed on CD4+CD25+ Tregs but not on CD4+CD25− conventional T cells. The difference in DcTRAIL R1 expression between the two T cell subsets may affect their responsiveness to TRAIL (4).

References

  1. Truneh, A. et al. (2000) J. Biol. Chem. 275:23319.
  2. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  3. de la Casa Esperon, E. et al. (2012) Epigenetics 7:626.
  4. Ikeda, T. et al. (2010) J. Immunol. 185:5259.

Long Name

Decoy TRAIL Receptor 1

Alternate Names

DcTRAIL R1, SOB, TNFRH1, TNFRSF23

Entrez Gene IDs

79201 (Mouse)

Gene Symbol

TNFRSF23

UniProt

Q9ER63

Additional DcTRAILR1/TNFRSF23 Products

Product Documents for Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

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Product Specific Notices for Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

For research use only

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