Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

Catalog # Availability Size / Price Qty
8479-DT-025

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Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse DcTRAIL R1/TNFSF23 is immobilized at 0.1 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% optimal binding response is approximately 2-12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse DcTRAIL R1/TNFRSF23 protein
Met30-Ser156, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met30
Predicted Molecular Mass
15 kDa
SDS-PAGE
23-30 kDa, reducing conditions

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8479-DT

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8479-DT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: DcTRAILR1/TNFRSF23

Decoy TRAIL receptor 1 (DcTRAIL R1), also called TNFRSF23 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein that binds TRAIL/TNFSF10 with high affinity (1). Mouse DcTRAIL R1 consists of a 29 amino acid (aa) signal peptide, a 126 aa mature region and a 21 aa GPI anchor signal. The mature region shows a TNFR cysteine rich domain (CRD) with three tandem A1/B2 repeats (2). Compared to signaling TRAIL receptors, DcTRAIL R1 does not trigger death signaling due to its lack of an intracellular death domain. Also, unlike many TNF receptors family members, DcTRAIL R1 does not contain a TRAFF-binding motif. Thus DcTRAIL R1 failed to induce NF kappa B activity. Structural studies revealed a strict species selectivity of the mouse DcTRAIL R1 for mouse and not human TRAIL based on amino acid specific electrostatic interactions between the two proteins (2). Mouse DcTRAIL R1, DcTRAIL R2 (TNFRSF22), and TNFRSF26 are encoded by clustered genes within the Kcnq1 domain of chromosome 7. The human counterpart of the Kcnq1 domain does not encode a functional homolog of the murine DcTRAIL R1 (3). Mouse DcTRAIL R1 is weakly expressed on CD4+CD25+ Tregs but not on CD4+CD25 conventional T cells. The difference in DcTRAIL R1 expression between the two T cell subsets may affect their responsiveness to TRAIL (4).

References
  1. Truneh, A. et al. (2000) J. Biol. Chem. 275:23319.
  2. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  3. de la Casa Esperon, E. et al. (2012) Epigenetics 7:626.
  4. Ikeda, T. et al. (2010) J. Immunol. 185:5259.
Long Name
Decoy TRAIL Receptor 1
Entrez Gene IDs
79201 (Mouse)
Alternate Names
APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR; DcTRAIL R1; DcTRAILR1; SOB; TNFRH1; TNFRSF23

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