Recombinant Mouse Dermatopontin Protein, CF Summary
Gln19-Val201 with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein) and EQ-1 (early quiescence-1), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature mouse Dermatopontin shares 92% and 97% amino acid sequence identity with human and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan Decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).
- Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
- Tzen, C.Y. and Y.W. Huang (2004) Exp. Cell Res. 294:30.
- Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
- Cronshaw, A.D. et al. (1993) Matrix 13:255.
- Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
- MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
- Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
- Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
- Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
- Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
- Okamoto, O. et al. (1996) J. Biochem. 119:106.
- Okamoto, O. et al. (1999) Biochem. J. 337:537.
- Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
- Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.
Citation for Recombinant Mouse Dermatopontin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Dermatopontin in Bone Marrow Extracellular Matrix Regulates Adherence but Is Dispensable for Murine Hematopoietic Cell Maintenance
Authors: AC Kramer, AL Blake, ME Taisto, MJ Lehrke, BR Webber, TC Lund
Stem Cell Reports, 2017;0(0):.
Sample Types: Whole Cells
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