Recombinant Mouse Dermatopontin Protein, CF

R&D Systems | Catalog # 5749-DP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Dermatopontin Protein (5749-DP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9QZZ6

Applications

Bioactivity
View all Dermatopontin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Dermatopontin protein
Gln19-Val201 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

22.8 kDa

SDS-PAGE

19-25 kDa, reducing conditions

Activity

Measured by its ability to modulate collagen fibrillogenesis.
20 µg/mL of rmDermatopontin can significantly enhance the rate of collagen fibrillogenesis.

Formulation, Preparation, and Storage

5749-DP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dermatopontin

Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein) and EQ-1 (early quiescence-1), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature mouse Dermatopontin shares 92% and 97% amino acid sequence identity with human and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan Decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).

References

  1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
  2. Tzen, C.Y. and Y.W. Huang (2004) Exp. Cell Res. 294:30.
  3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
  4. Cronshaw, A.D. et al. (1993) Matrix 13:255.
  5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
  6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
  7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
  8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
  9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
  10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
  11. Okamoto, O. et al. (1996) J. Biochem. 119:106.
  12. Okamoto, O. et al. (1999) Biochem. J. 337:537.
  13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
  14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.

Alternate Names

DPT, Eq1

Entrez Gene IDs

1805 (Human); 56429 (Mouse); 289178 (Rat)

Gene Symbol

DPT

UniProt

Q9QZZ6 (Mouse)

Additional Dermatopontin Products

Product Documents for Recombinant Mouse Dermatopontin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Dermatopontin Protein, CF

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Recombinant Mouse Dermatopontin Protein, CF

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