Recombinant Mouse Dermatopontin Protein, CF

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5749-DP-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Dermatopontin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to modulate collagen fibrillogenesis. 20 µg/mL of rmDermatopontin can significantly enhance the rate of collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived mouse Dermatopontin protein
Gln19-Val201 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln19 predicted
Predicted Molecular Mass
22.8 kDa
SDS-PAGE
23 kDa, reducing conditions

Product Datasheets

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5749-DP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5749-DP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Dermatopontin

Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein) and EQ-1 (early quiescence-1), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature mouse Dermatopontin shares 92% and 97% amino acid sequence identity with human and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan Decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).

References
  1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
  2. Tzen, C.Y. and Y.W. Huang (2004) Exp. Cell Res. 294:30.
  3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
  4. Cronshaw, A.D. et al. (1993) Matrix 13:255.
  5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
  6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
  7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
  8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
  9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
  10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
  11. Okamoto, O. et al. (1996) J. Biochem. 119:106.
  12. Okamoto, O. et al. (1999) Biochem. J. 337:537.
  13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
  14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.
Entrez Gene IDs
1805 (Human); 56429 (Mouse); 289178 (Rat)
Alternate Names
Dermatopontin; DPT; Eq1; TRAMP; Tyrosine-rich acidic matrix protein

Citation for Recombinant Mouse Dermatopontin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dermatopontin in Bone Marrow Extracellular Matrix Regulates Adherence but Is Dispensable for Murine Hematopoietic Cell Maintenance
    Authors: AC Kramer, AL Blake, ME Taisto, MJ Lehrke, BR Webber, TC Lund
    Stem Cell Reports, 2017-08-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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