Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have a high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The mouse DPP7 precursor consists of a signal peptide (amino acids (aa) 1-33) and a mature chain (aa 34-506). The purified recombinant mouse DPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under otherwise identical conditions.
Recombinant Mouse DPP7 Protein, CF
R&D Systems | Catalog # 3436-SE
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse DPP7 Protein (3436-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse DPPII/QPP/DPP7 protein
Asp34-Arg506, with a C-terminal 10-His tag
Asp34-Arg506, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp34
Predicted Molecular Mass
54 kDa
SDS-PAGE
65 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC (KP-AMC).
The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
3436-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 200 μg/mL in 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: DPPII/QPP/DPP7
References
- Araki, H. et al. (2001) J. Biochem. 129:279.
- Maes, M.-B. et al. (2005) Biochem. J. 386:315.
- Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
- Lankas, G.R. et al. (2005) Diabetes 54:2988.
Long Name
Dipeptidyl-peptidase II
Alternate Names
DPP7, QPP
Gene Symbol
DPP7
UniProt
Additional DPPII/QPP/DPP7 Products
Product Documents for Recombinant Mouse DPP7 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse DPP7 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Mouse DPP7 Protein, CF (3436-SE):
Materials
- Assay Buffer: 25 mM MES, pH 6.0
- Recombinant Mouse DPPII/QPP/DPP7 (rmDPPII) (Catalog # 3436-SE)
- Substrate: Lys-Pro-AMC (Bachem, Catalog # 1745), 10 mM stock solution in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmDPPII to 0.04 ng/µL in Assay Buffer.
- Dilute Substrate to 200 µM in Assay Buffer.
- Load into plate 50 µL of 0.04 ng/µL rmDPPII, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).
- rmDPPII: 0.002 µg
- Substrate: 100 µM