Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein

Ectodysplasin is a 45 kDa type II transmembrane TNF superfamily protein that is associated with X-linked hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and sweat gland development (1 - 4). The mouse EDA-A1 cDNA encodes a 41 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 329 extracellular region that contains a terminal TNF homology domain, a collagenous domain, and a stalk region (5 - 8). Within the collagenous and TNF homology domains, mouse EDA-A1 shares greater than 97% aa  sequence identity with bovine, canine, human, and rat EDA-A1. Multiple alternately spliced EDA variants have been described (4, 9). The dominant variant, EDA-A2, has a deletion of two amino acids that changes the receptor binding selectivity from EDAR to XEDAR (4, 9, 10). The collagenous domain of EDA-A1 mediates noncovalent homotrimer formation (7, 8). Shedding of the collagenous and TNF homology domains of EDA-A1 is accomplished by a furin-like protease. The released fragment maintains its trimeric state and ability to bind EDAR (11, 12). Some EDA-A1 polymorphisms found in HED patients alter the protease recognition site and prevent shedding (11). EDA-A1 is expressed in developing hair follicles, epidermis, teeth, sweat glands, salivary glands, and forebrain (8, 10, 13 - 15). It regulates ectodermal appendage formation and is critical to the patterning and morphogenesis of hair follicles, partially through the induction of Lymphotoxin beta (7, 14, 16). Receptor and ligand expression are regulated by factors involved in many aspects of tissue morphgenesis. EDA-A1 expression is induced by Wnt6, (14, 15) while the expression of EDAR is induced by Activin  beta A and inhibited by BMP-2, -4, and -7 (15, 17).