Recombinant Mouse EphA7 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
608-A7-200
R&D Systems Recombinant Proteins and Enzymes
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Citations (16)
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Recombinant Mouse EphA7 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphA7 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant human Ephrin-A4 Fc Chimera with a linear range of 0.16 ‑ 10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse EphA7 protein
Mouse EphA7
(Ala30-Pro549)
Accession # JC5673
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.7 kDa (monomer)
SDS-PAGE
110 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

608-A7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: EphA7

EphA7, also known as Mdk1, Hek11, Ehk3, Ebk, and Cek11, is a 115-120 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA7 preferentially binds to and is activated by Ephrin-A1, -A2, -A3, -A4, and -A5 (3 - 6). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 527 amino acid (aa) extracellular domain (ECD) of mouse EphA7 contains an N‑terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 421 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (7). Within the ECD, mouse EphA7 shares 98% and 99% aa sequence identity with human and rat EphA7, respectively. Alternate splicing generates a differentially expressed kinase-defective isoform of mouse EphA7 that lack most of the cytoplasmic domain and a soluble isoform that is truncated following the first FnIII domain (4, 6 - 9). In mouse, EphA7 is expressed in discrete regions of the developing and adult neocortex (4, 7 - 10), Purkinje layer of the cerebellum (7), limbic system (4, 8 ‑ 10), visual and auditory systems (7, 8, 11), and the peripheral sensory nervous system (7, 8). EphA7 functions as a repulsive guidance molecule during the targeting of retinal axons to the superior colliculus  and of neocortical axons to the thalamus (4, 10, 11). EphA7 is also expressed in mesenchymal cells along routes of axon innervation during limb development (12). EphA7 ligands are expressed in a complementary pattern during embryogenesis (3, 10 - 12). EphA7 is selectively expressed in early stages of the B cell lineage, and a 50 kDa secreted form is produced by mature peripheral B and T cells (6, 13). This isoform is also expressed in human lung carcinoma (14). EphA7 can be up or downregulated in a variety of human cancers (6, 15, 16).

References
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Ciossek, T. and A. Ullrich (1997) Oncogene 14:35.
  4. Miller, K. et al. (2006) J. Comp. Neurol. 496:627.
  5. Gale, N.W. et al. (1996) Neuron 17:9.
  6. Dawson, D.W. et al. (2007) Oncogene 26:4243.
  7. Ciossek, T. et al. (1995) Oncogene 9:97.
  8. Mori, T. et al. (1995) Mol. Brain Res. 34:154.
  9. Ciossek, T. et al. (1999) Mol. Brain Res. 74:231.
  10. Torii, M. and P. Levitt (2005) Neuron 48:563.
  11. Rashid, T. et al. (2005) Neuron 47:57.
  12. Araujo, M. et al. (1998) Development 125:4195.
  13. Aasheim, H.-C. et al. (1997) Blood 90:3613.
  14. Tsuboi, M. et al. (2010) Int. J. Oncol. 36:635.
  15. Hafner, C. et al. (2004) Clin. Chem. 3:490.
  16. Wang, J. et al. (2007) Hum. Pathol. 38:1649.
Long Name
Eph Receptor A7
Entrez Gene IDs
2045 (Human); 13841 (Mouse)
Alternate Names
Cek11; Ebk; EC 2.7.10; EC 2.7.10.1; Ehk3; EHK-3; EK11; EPH homology kinase 3; Eph homology kinase-3; EPH receptor A7; EphA7; EPH-like kinase 11; ephrin type-A receptor 7; Hek11; Mdk1; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3

Citations for Recombinant Mouse EphA7 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. EphA7 promotes myogenic differentiation via cell-cell contact
    Authors: LL Arnold, A Cecchini, DA Stark, J Ihnat, RN Craigg, A Carter, S Zino, D Cornelison
    Elife, 2020;9(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Erythropoietin-producing hepatocellular A7 triggering ovulation indicates a potential beneficial role for polycystic ovary syndrome
    Authors: S Li, J Zhai, J Liu, F Di, Y Sun, W Li, ZJ Chen, Y Du
    EBioMedicine, 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme.
    Authors: Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A
    Development, 2014;141(17):3420-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells.
    Authors: Defourny J, Poirrier A, Lallemend F, Mateo Sanchez S, Neef J, Vanderhaeghen P, Soriano E, Peuckert C, Kullander K, Fritzsch B, Nguyen L, Moonen G, Moser T, Malgrange B
    Nat Commun, 2013;4(0):1438.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development.
    Authors: Park, E, Kim, Y, Noh, H, Lee, H, Yoo, S, Park, S
    Cell Death Differ, 2013;20(1):169-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
    Authors: Dewannieux M, Vernochet C, Ribet D, Bartosch B, Cosset FL, Heidmann T
    PLoS Pathog., 2011;7(10):e1002309.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Pro-neurotrophins secreted from retinal ganglion cell axons are necessary for ephrinA-p75NTR-mediated axon guidance.
    Authors: Marler KJ, Poopalasundaram S, Broom ER
    Neural Dev, 2010;5(0):30.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Ephrins negatively regulate cell proliferation in the epidermis and hair follicle.
    Authors: Genander M, Holmberg J, Frisen J
    Stem Cells, 2010;28(7):1196-205.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling.
    Authors: Torii M, Hashimoto-Torii K, Levitt P, Rakic P
    Nature, 2009;461(7263):524-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
    Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
    Neuron, 2008;59(5):746-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
    Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
    Cell, 2007;129(2):359-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
    Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
    Biochemistry, 2007;46(7):1799-810.
    Species: Human
    Sample Types: Peptide
    Applications: Binding Assay
  14. Opposing gradients of ephrin-As and EphA7 in the superior colliculus are essential for topographic mapping in the mammalian visual system.
    Authors: Rashid T, Upton AL, Blentic A, Ciossek T, Knoll B, Thompson ID, Drescher U
    Neuron, 2005;47(1):57-69.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Ephrin-A5 inhibits growth of embryonic sensory neurons.
    Authors: Munoz LM, Zayachkivsky A, Kunz RB, Hunt JM, Wang G, Scott SA
    Dev. Biol., 2005;283(2):397-408.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: ICC
  16. Targeting the EphA4 receptor in the nervous system with biologically active peptides.
    Authors: Murai KK, Nguyen LN, Koolpe M, McLennan R, Krull CE, Pasquale EB
    Mol. Cell. Neurosci., 2003;24(4):1000-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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