Recombinant Mouse EphA8 Fc Chimera Protein, CF

R&D Systems | Catalog # 454-A8

R&D Systems
Discontinued Product
454-A8 has been discontinued. View all EphA8 products.

Key Product Details

Source

NS0

Accession Number

O09127

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all EphA8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EphA8 protein
Mouse EphA8
(Gly27 - Arg540)
Accession # O09127		 DIEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly27

Predicted Molecular Mass

84.2 kDa (monomer)

SDS-PAGE

100-120 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse EphA8 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant human Ephrin-A5 Fc Chimera with a linear range of
0.31-20 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

454-A8
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, PEG, CHAPS and Imidazole.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA8

EphA8, also known as Hek3 and Eek, is a 120 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA4 binds and is activated by class A Ephrins but not class B Ephrins (3, 4). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 514 amino acid (aa) extracellular domain (ECD) of mouse EphA8 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 442 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (3). Within the ECD, mouse EphA8 shares 97% and 99% aa sequence identity with human and rat EphA8, respectively. EphA8 is expressed in neurons in the mesencephalon of the developing brain, particularly in the rostral tectum and the superior colliculus (5 - 7). It is enriched at the tips of neurite processes and plays a role in projection of superior colliculus axons through the posterior commissure (6, 8). Its expression enhances neurite extension be means of a mechanism that does not require catalytic activity of the tyrosine kinase domain (9). Ephrin-mediated activation of the EphA8 kinase induces phosphorylation of tyrosine residues in the cytoplasmic domain, leading to association with signaling and scaffolding proteins and inhibition of cell-cell adhesion (4, 8, 10). Ligand binding can also promote the Integrin-mediated cellular adhesion to Fibronectin (11). This function, like the enhancement of neurite extension, does not require activation of the kinase domain (11).

References

  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Park, S. and M.P. Sanchez (1997) Oncogene 14:533.
  4. Choi, S. et al. (1999) Mol. Cells 9:440.
  5. Koo, J. et al. (2003) Dev. Dyn. 226:596.
  6. Park, S. et al. (1997) EMBO J. 16:3106.
  7. Shim, S. et al. (2007) Mol. Cell. Biol. 27:1614.
  8. Shin, J. et al. (2007) Mol. Cell. Biol. 27:8113.
  9. Gu, C. et al. (2005) Oncogene 24:4243.
  10. Choi, S. and S. Park (1999) Oncogene 18:5413.
  11. Gu, C. and S. Park (2001) Mol. Cell. Biol. 21:4579.

Long Name

Eph Receptor A8

Alternate Names

Eek, Hek3

Entrez Gene IDs

2046 (Human); 13842 (Mouse)

Gene Symbol

EPHA8

UniProt

O09127

Additional EphA8 Products

Product Documents for Recombinant Mouse EphA8 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse EphA8 Fc Chimera Protein, CF

For research use only

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