Recombinant Mouse EphB2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
467-B2-200
Product Details
Citations (14)
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Recombinant Mouse EphB2 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmEphB2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-B2/Fc Chimera with a linear range of 0.039-2.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse EphB2 protein
Mouse EphB2
(Val27-Lys548)
Accession # P54763
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.3 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

467-B2

Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: EphB2

EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name
Eph Receptor B2
Entrez Gene IDs
2048 (Human); 13844 (Mouse)
Alternate Names
CAPB; Cek5; Drt; DRTEphB2; EC 2.7.10; EC 2.7.10.1; EK5; elk-related tyrosine kinase; EPH receptor B2; eph tyrosine kinase 3; EphB2; EPH-like kinase 5; ephrin type-B receptor 2; EPHT3MGC87492; EPTH3; Erk; ERKHek5; Hek5; Nuk; PCBC; protein-tyrosine kinase HEK5; Qek2; Renal carcinoma antigen NY-REN-47; Sek3; Tyro5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5

Citations for Recombinant Mouse EphB2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
    Authors: Muto A, Yi T, Harrison KD, Davalos A, Fancher TT, Ziegler KR, Feigel A, Kondo Y, Nishibe T, Sessa WC, Dardik A
    J. Exp. Med., 2011;208(3):561-75.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Ephrins negatively regulate cell proliferation in the epidermis and hair follicle.
    Authors: Genander M, Holmberg J, Frisen J
    Stem Cells, 2010;28(7):1196-205.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. EphB signaling controls lineage plasticity of adult neural stem cell niche cells.
    Authors: Nomura T, Goritz C, Catchpole T, Henkemeyer M, Frisen J
    Cell Stem Cell, 2010;7(6):730-43.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. nev (cyfip2) is required for retinal lamination and axon guidance in the zebrafish retinotectal system.
    Authors: Pittman AJ, Gaynes JA, Chien CB
    Dev. Biol., 2010;344(2):784-94.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC
  5. Blockade of EphB2 enhances neurogenesis in the subventricular zone and improves neurological function after cerebral cortical infarction in hypertensive rats.
    Authors: Xing S, He Y, Ling L, Hou Q, Yu J, Zeng J, Pei Z
    Brain Res., 2008;1230(0):237-46.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. Temporal regulation of ephrin/Eph signalling is required for the spatial patterning of the mammalian striatum.
    Authors: Passante L, Gaspard N, Degraeve M, Frisen J, Kullander K, De Maertelaer V, Vanderhaeghen P
    Development, 2008;135(19):3281-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo.
    Authors: Lim BK, Matsuda N, Poo MM
    Nat. Neurosci., 2008;11(2):160-9.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  8. Divergent roles for Eph and ephrin in avian cranial neural crest.
    Authors: Mellott DO, Burke RD
    BMC Dev. Biol., 2008;8(0):56.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  9. Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
    Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
    Biochemistry, 2007;46(7):1799-810.
    Species: Human
    Sample Types: Peptide
    Applications: Binding Assay
  10. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006;119(0):559-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
    Authors: Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J
    Cell, 2006;125(6):1151-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006;281(43):32574-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Ephrin-B1 forward and reverse signaling are required during mouse development.
    Authors: Davy A, Aubin J, Soriano P
    Genes Dev., 2004;18(5):572-83.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  14. Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
    Authors: Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
    Nat. Neurosci., 2003;6(2):153-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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