Recombinant Mouse FAM3C Protein, CF

R&D Systems | Catalog # 2868-FM

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse FAM3C Protein (2868-FM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q91VU0

Applications

Bioactivity
View all FAM3C Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FAM3C protein
Gln25-Asp227, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln25 predicted, sequencing might be blocked

Predicted Molecular Mass

23 kDa

SDS-PAGE

23-25 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells.
The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation, and Storage

2868-FM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FAM3C

Family with sequence similarity 3, member C (FAM3C), also known as Interleukin-Like EMT Inducer (ILEI), is an approximately 25 kDa secreted protein in the FAM group of molecules (1, 2). Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. Mature mouse FAM3C shares 94% and 97% amino acid sequence identity with human and rat FAM3C, respectively. It is widely expressed, notably in the nonsensory epithelium of auditory semicircular canals (1) and in the photoreceptor layer of the retina (3). FAM3C is up‑regulated and shows altered intracellular localization in various tumors (4, 5). Its translation is normally silenced by the binding of hnRNP E1 to the FAM3C mRNA, but this inhibition is removed following cellular exposure to TGF-beta (6, 7). FAM3C, like TGF-beta, promotes epithelial-mesenchymal transition (EMT), tumor growth, and metastasis (4-6). FAM3C cooperates with oncogenic Ras to promote the up‑regulation of PDGF-C and PDGF R beta, leading to increased PDGF receptor signaling (5). R&D Systems in-house testing indicates that FAM3C supports the adhesion of Caki cells.

References

  1. Pilipenko, V.V. et al. (2004) Gene 335:159.
  2. Zhu, Y. et al. (2002) Genomics 80:144.
  3. Katahira, T. et al. (2010) Biochem. Biophys. Res. Commun. 392:301.
  4. Waerner, T. et al. (2006) Cancer Cell 10:227.
  5. Lahsnig, C. et al. (2009) Oncogene 28:638.
  6. Chaudhury, A. et al. (2010) Nat. Cell Biol. 12:286.
  7. Hussey, G.S. et al. (2011) Mol. Cell 41:419.

Long Name

Family With Sequence Similarity 3, Member C

Alternate Names

D6WSU176e, ILEI

Entrez Gene IDs

10447 (Human); 27999 (Mouse); 312159 (Rat)

Gene Symbol

FAM3C

UniProt

Q91VU0

Additional FAM3C Products

Product Documents for Recombinant Mouse FAM3C Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse FAM3C Protein, CF

For research use only

Related Research Areas

Diabetes

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