Home / FGL2 / Recombinant Mouse FGL2 Protein, CF

Recombinant Mouse FGL2 Protein, CF

Catalog #: 5257-FL
6 Citations Datasheet / COA / SDS
Newer Version Available: 10691-FL
NEW
Item is in-process of being discontinued and will be replaced with rmFGL2 with a HA tag, Catalog # 10691-FL

Discontinued Product

5257-FL has been discontinued and is replaced by 10691-FL.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (6)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FGL2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of mouse splenic T lymphocytes. Chan, C.W. et al. (2003) J. Immunol. 170:4036. The soluble Recombinant Mouse FGL2 inhibited the proliferation with a typical ED50 value of 1-4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse FGL2 protein
Val20-Pro432 & Asp31-Pro432 both with a C-terminal 6-His tag
Accession #
P12804
N-terminal Sequence
Analysis
Val20 & Asp31
Structure / Form
Disulfide-linked homotetramer
Predicted Molecular Mass
47.7 kDa (monomer)
SDS-PAGE
55-80 kDa, reducing conditions

Product Datasheets

You must select a language.

x

5257-FL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5257-FL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FGL2

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64-70 kDa secreted glycoprotein of the Fibrinogen-like superfamily. It has prothrombinase activity and also promotes T regulatory (Treg) activity (1-6). The mouse FGL2 gene encodes a 432 amino acid (aa) protein that contains a 19 aa signal sequence and a 413 aa mature sequence with a coiled-coil region and a fibronectin C-terminal homology domain or FRED (1, 2). A 260-280 aa FGL2 complex is thought to be a tetramer formed by covalent disulfide linkage of dimers that are asoociated via coiled-coil interactions (2, 3). Mature mouse FGL2 shares 91% aa identity with rat FGL2, and 77-80% aa identity with human, equine, porcine, bovine and canine FGL2. FGL2 appears to have two modes of action. One mode involves its prothrombinase activity, which requires calcium and acidic phospholipids (4). This mode is thought to be active during hepatitis viral infections when FGL2, produced by macrophages in response to IFN-gamma, induces hepatic apoptosis and fibrin deposition (7). In addition, FGL2 produced by endothelial cells in response to TNF-alpha within cardiac xenografts or allografts promotes coagulation during acute vascular rejection (7-9). A second mode of action involves soluble (not phospholipid-associated) FGL2 and is independent of prothrombinase activity (2). Soluble FGL2 is required for Treg function, and directly suppresses DC, T, and B cell immune reactivity; consequently, some FGL2-deficent mice develop autoimmune glomerulonephritis (5, 6). In vitro, soluble FGL2 can skew T cell polarization toward Th2 and inhibit proliferation of stimulated T cells and maturation of DC (6). In pregnancy, fetal trophoblast cells secrete FGL2. The immune suppressive mode of FGL2 may prevent early fetal loss; however, the procoagulant mode is thought to mediate infection-triggered abortion (10).

References
  1. Koyama, T. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1609.
  2. Marazzi, S. et al. (1998) J. Immunol.161:138.
  3. Olson, G. E. et al. (2004) J. Biol. Chem. 279:51266.
  4. Chan, C. W. Y. et al. (2002) J. Immunol. 168:5170.
  5. Shalev, I. et al. (2008) J. Immunol.180:249.
  6. Chan, C. W. Y. et al. (2003) J. Immunol. 170:4036.
  7. Liu, M. et al. (2006) J. Immunol. 176:7028.
  8. Mendicino, M. et al. (2005) Circulation 112:248.
  9. Ning, Q. et al. (2005) J. Immunol. 174:7403.
  10. Clark, D. A. et al. (2004) Mol. Hum. Reprod. 10:99.
Long Name
Fibrinogen-like 2
Entrez Gene IDs
10875 (Human); 14190 (Mouse); 84586 (Rat); 102123631 (Cynomolgus Monkey)
Alternate Names
FGL2; fibrinogen-like 2; Fibrinogen-like protein 2; Fibroleukin; pT49T49; T49

Citations for Recombinant Mouse FGL2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Local administration of regulatory T cells promotes tissue healing
    Authors: Nayer, B;Tan, JL;Alshoubaki, YK;Lu, YZ;Legrand, JMD;Lau, S;Hu, N;Park, AJ;Wang, XN;Amann-Zalcenstein, D;Hickey, PF;Wilson, T;Kuhn, GA;Müller, R;Vasanthakumar, A;Akira, S;Martino, MM;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells
    Authors: Q Zhao, J Hu, L Kong, S Jiang, X Tian, J Wang, R Hashizume, Z Jia, NW Fowlkes, J Yan, X Xia, SF Yi, LH Dao, D Masopust, AB Heimberger, S Li
    Nature Communications, 2023-02-10;14(1):735.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2
    Authors: Y Fu, Y Ding, Q Wang, F Zhu, Y Tan, X Lu, B Guo, Q Zhang, Y Cao, T Liu, L Cui, W Xu
    Sci Adv, 2020-02-26;6(9):eaay9269.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Cell Culture, In Vivo
  4. Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5
    Authors: Y Zhou, J Lei, Q Xie, L Wu, S Jin, B Guo, X Wang, G Yan, Q Zhang, H Zhao, J Zhang, X Zhang, J Wang, J Gu, X Liu, D Ye, H Miao, CN Serhan, Y Li
    Sci Adv, 2019-11-13;5(11):eaax0629.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation
    Authors: J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li
    Nat Commun, 2019-01-25;10(1):448.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Stroma-derived Fibrinogen-like Protein 2 Activates Cancer-associated Fibroblasts to Promote Tumor Growth in Lung Cancer
    Authors: Y Zhu, L Zhang, H Zha, F Yang, C Hu, L Chen, B Guo, B Zhu
    Int. J. Biol. Sci., 2017-06-01;13(6):804-814.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse FGL2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse FGL2 Protein, CF and earn rewards!

Have you used Recombinant Mouse FGL2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review