Recombinant Mouse Follistatin-like 5/FSTL5 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival. The ED50 for this effect is 2-10 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived mouse Follistatin-like 5/FSTL5 protein
    Arg21-Ala847, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg21
  • Predicted Molecular Mass
    94 kDa
  • SDS-PAGE
    85-98 kDa, reducing conditions
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9065-FN
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 500 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Mouse Follistatin-like 5/FSTL5 (Catalog #
9065-FN) inhibits Hep3B human hepatocellular carcinoma cell survival. The ED50 for this effect is typicallly
2-10 μg/mL.
Background: Follistatin-like 5/FSTL5
Follistatin-like 5 (FSTL5) is a secreted 94 kDa (predicted) member of the SPARC protein family (1). In its N-terminal half, FSTL5 contains a Kazal-like domain, two EF-hand domains, and two Ig-like domains. Mature mouse FSTL5 shares 91% and 97% amino acid sequence identity with human and rat FSTL5, respectively. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and downregulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).
  • References:
    1. Bradshaw, A.D. (2012) Int. J. Biochem. Cell Biol. 44:480.
    2. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
    3. Masuda, T. et al. (2014) Congenit. Anom. (Kyoto) 54:63.
    4. Zhang, D. et al. (2015) Int. J. Clin. Exp. Pathol. 8:3386.
    5. Remke, M. et al. (2011) J. Clin. Oncol. 29:3852.
  • Entrez Gene IDs:
    56884 (Human); 213262 (Mouse); 365823 (Rat)
  • Alternate Names:
    Follistatin like 5; Follistatin-like 5; Follistatin-like protein 5; follistatin-related protein 5; FSL5; FSTL5; KIAA1263DKFZp566D234
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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