Recombinant Mouse Galectin-2 Protein, CF

R&D Systems | Catalog # 8579-GA

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Mouse Galectin-2 Protein (8579-GA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AAH27504

Applications

Bioactivity
View all Galectin-2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse Galectin-2 protein
Ser2-Glu130

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

15 kDa

SDS-PAGE

12-14 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells.
The ED50 for this effect is 0.5-2.5 μg/mL.

Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385.
The ED50 for this effect is 2.5-12.5 μg/mL.

Formulation, Preparation, and Storage

8579-GA
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
Reconstitution

Reconstitute at 400 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-2

Galectin-2 is an approximately 14 kDa, homodimeric carbohydrate-binding protein of the S-type lectin family (1, 2). Like other prototype Galectins, Galectin-2 consists of a single carbohydrate-recognition domain (CRD) (3). Mouse Galectin-2 shares 86% and 66% amino acid sequence identity with rat and human Galectin-2, respectively. Galectin-2 is primarily expressed in the digestive tract and skin of mice (4-6). It can be S-nitrosylated with S-nitrosoglutathione in mouse gastric mucosa (7).
S-nitrosylation prevents Galectin-2 inactivation by hydrogen peroxide, suggesting that this modification protects Galectin-2 function against oxidative stress (8). Galectin-2 appears to have a role in immune responses. Galectin-2 mRNA and protein levels are down-regulated following infection with H. felis (9). Additionally, Galectin-2 treatment has been shown to reduce MHC class I-restricted immune responses and ameliorate acute and chronic experimental colitis in mice by inducing apoptosis of T cells (6, 10). Galectin-2 may also play a role in cancer since circulating levels of human Galectin-2 are elevated in colon and breast cancer patients (11).

References

  1. Hokama, A. et al. (2008) World J. Gastroenterol. 14:5133.
  2. Barondes, S.H. et al. (1994) Cell 76:597.
  3. Hirabayashi, J. and K. Kasai (1993) Glycobiology 3:297.
  4. Nio, J. et al. (2005) J. Histochem. Cytochem. 53:1323.
  5. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
  6. Loser, K. et al. (2009) J. Immunol. 182:5419.
  7. Ohtake, K. et al. (2009) J. Proteomics 72:750.
  8. Tamura, M. et al. (2015) Biochem. Biophys. Res. Commun. 457:712.
  9. Takaishi, S. and T.C. Wang (2007) Cancer Sci. 98:284.
  10. Paclik, D. et al. (2008) J. Mol. Med. 86:1395.
  11. Chen, C. et al. (2014) Br. J. Cancer 110:741.

Alternate Names

GAL2, Galectin2, LGALS2

Entrez Gene IDs

3957 (Human); 107753 (Mouse); 171134 (Rat)

Gene Symbol

LGALS2

UniProt

AAH27504

Additional Galectin-2 Products

Product Documents for Recombinant Mouse Galectin-2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Galectin-2 Protein, CF

For research use only

Related Research Areas

Lectins
NKG2D Receptors and Ligands

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