Recombinant Mouse Gas6 Protein

Formulations:
Catalog # Availability Size / Price Qty
986-GS-025
Product Details
Citations (21)
FAQs
Reviews (3)

Recombinant Mouse Gas6 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 20-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Gas6 protein
Asp115-Pro673, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp115
Predicted Molecular Mass
62 kDa
SDS-PAGE
72 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

986-GS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

986-GS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.

Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

References
  1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  2. Goruppi, S. et al. (1996) Oncogene 12:471.
  3. Crosier, K and P. Crosier (1997) Pathology 29:131.
  4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
  5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Citations for Recombinant Mouse Gas6 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Respiratory syncytial virus infection exacerbates pneumococcal pneumonia via Gas6/Axl-mediated macrophage polarization
    Authors: T Shibata, A Makino, R Ogata, S Nakamura, T Ito, K Nagata, Y Terauchi, T Oishi, M Fujieda, Y Takahashi, M Ato
    J. Clin. Invest., 2020;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Gas6 Attenuates Sepsis-Induced Tight Junction Injury and Vascular Endothelial Hyperpermeability via the Axl/NF-kappaB Signaling Pathway
    Authors: J Ni, M Lin, Y Jin, J Li, Y Guo, J Zhou, G Hong, G Zhao, Z Lu
    Front Pharmacol, 2019;10(0):662.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Gas6/Axl signaling attenuates alveolar inflammation in ischemia-reperfusion-induced acute lung injury by up-regulating SOCS3-mediated pathway
    Authors: CK Peng, CP Wu, JY Lin, SC Peng, CH Lee, KL Huang, CH Shen
    PLoS ONE, 2019;14(7):e0219788.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. TAM receptors attenuate murine NK-cell responses via E3 ubiquitin ligase Cbl-b
    Authors: LM Chirino, S Kumar, M Okumura, DE Sterner, M Mattern, TR Butt, T Kambayashi
    Eur. J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Gas6/Axl signaling attenuates alveolar inflammation in ischemia-reperfusion-induced acute lung injury by up-regulating SOCS3-mediated pathway
    Authors: CK Peng, CP Wu, JY Lin, SC Peng, CH Lee, KL Huang, CH Shen
    PLoS ONE, 2019;14(7):e0219788.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Gas6 Prevents Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells via Production of PGE2, PGD2 and Their Receptors
    Authors: J Jung, YJ Lee, YH Choi, EM Park, HS Kim, JL Kang
    Cells, 2019;8(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. MERTK mediated novel site Akt phosphorylation alleviates SAV1 suppression
    Authors: Y Jiang, Y Zhang, JY Leung, C Fan, KI Popov, S Su, J Qian, X Wang, A Holtzhause, E Ubil, Y Xiang, I Davis, NV Dokholyan, G Wu, CM Perou, WY Kim, HS Earp, P Liu
    Nat Commun, 2019;10(1):1515.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
    Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
    Neuron, 2018;97(5):1023-1031.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Stromal Gas6 promotes the progression of premalignant mammary cells
    Authors: AM Gomes, EC Carron, KL Mills, AM Dow, Z Gray, CR Fecca, MA Lakey, P Carmeliet, F Kittrell, D Medina, HL Machado
    Oncogene, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
    Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
    Neuron, 2017;94(3):500-516.e9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
    Authors: Lee Y, Park H, Woo S, Park E, Kang J
    J Pharmacol Exp Ther, 2014;350(3):563-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. TAM receptors affect adult brain neurogenesis by negative regulation of microglial cell activation.
    Authors: Ji R, Tian S, Lu H, Lu Q, Zheng Y, Wang X, Ding J, Li Q, Lu Q
    J Immunol, 2013;191(12):6165-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
    Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
    Immunology, 2012;135(1):40-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. An anti-Axl monoclonal antibody attenuates xenograft tumor growth and enhances the effect of multiple anticancer therapies.
    Authors: Ye X, Li Y, Stawicki S
    Oncogene, 2010;29(38):5254-64.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  16. Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice.
    Authors: Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A
    J. Hepatol., 2009;51(1):55-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
    Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
    J. Immunol., 2009;183(5):3004-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
    Authors: Uehara H, Shacter E
    J. Immunol., 2008;180(4):2522-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  19. Role of Gas6 in erythropoiesis and anemia in mice.
    Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
    J. Clin. Invest., 2008;118(2):583-96.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  20. A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
    Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
    Blood, 2007;109(3):1026-33.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  21. TAM receptors are pleiotropic inhibitors of the innate immune response.
    Authors: Rothlin CV, Ghosh S, Zuniga EI, Oldstone MB, Lemke G
    Cell, 2007;131(6):1124-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse Gas6 Protein

Average Rating: 2.3 (Based on 3 Reviews)

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Recombinant Mouse Gas6 Protein
By Anonymous on 10/09/2019
Application: In vitro bioactivity in cell culture

Recombinant Mouse Gas6 Protein
By Anonymous on 02/02/2017
Application: Other

We now need to make our own recombinant protein as this one will not be suitable for studies we are doing.

R&D Systems response: Thank you so much for taking the time to share your experience using R&D Systems Proteins. We value your opinion and use our online reviews as testimonials from our customers of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when our customers are unhappy. We have reached out to offer additional support with this product and are unable to reach you. Please contact a technical service representative at techsupport@bio-techne.com and allow us to further troubleshoot some of the concerns you have mentioned in your online review. We look forward to hearing from you.


Recombinant Mouse Gas6 Protein
By Anonymous on 08/15/2016
Application: In vitro bioactivity in cell culture