Recombinant Mouse GDF-7 Protein

R&D Systems | Catalog # 779-G7

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse GDF-7 Protein (779-G7)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

NP_038555

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all GDF-7/BMP-12 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse GDF-7/BMP-12 protein
Thr308-Arg453

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr308

Predicted Molecular Mass

15 kDa

SDS-PAGE

28 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.25-1.25 µg/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 779-G7
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCL containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 779-G7/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-7/BMP-12

Growth Differentiation Factor 7 (GDF-7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP-3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. Mature GDF-7 contains 146 aa residues with the characteristic seven conserved cysteine residues. The bioactive GDF-7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF-5, GDF-6 and GDF-7, which share 80 - 86% identity with each other, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-7 are 98% identical. It has been reported that GDF-7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF-7 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activiation of Smad proteins.

References

  1. Storm, E.E., et al. (1994) Nature 368:639.
  2. Nishitoh, H., et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H., et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.

Long Name

Growth Differentiation Factor 7

Alternate Names

BMP-12, GDF7

Entrez Gene IDs

151449 (Human); 238057 (Mouse)

Gene Symbol

GDF7

UniProt

NP_038555

Additional GDF-7/BMP-12 Products

Product Documents for Recombinant Mouse GDF-7 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 779-G7 (with carrier)
Download SDS 779-G7/CF (carrier free)

Product Specific Notices for Recombinant Mouse GDF-7 Protein

For research use only

Citations for Recombinant Mouse GDF-7 Protein

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