Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

R&D Systems | Catalog # 9844-GR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse GFR alpha-like Fc Chimera Protein (9844-GR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q6SJE0-1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all GFR alpha-like Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse GFR alpha-like protein
Mouse GFR alpha-like
(Gln20-Gly349)
Accession # Q6SJE0-1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Thr21

Predicted Molecular Mass

64 kDa

SDS-PAGE

66-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse GFR alpha ‑like Fc Chimera is coated at 0.1 μg/mL, 100 μL/well, it binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.

Scientific Data Images for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity

Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity

When Recombinant Mouse GFR alpha ‑like Fc Chimera (Catalog # 9844-GR) is immobilized at 0.1 μg/mL, 100 μL/well, It binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.

Formulation, Preparation, and Storage

9844-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GFR alpha-like

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

References


  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Yang, L. et al. (2017) Nature Medicine 23:1158.
  3. Mullican, S. et al. (2017) Nature medicine 23:1150.

  4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha-like

Alternate Names

GFR alpha like, GFRAL, GRAL

Entrez Gene IDs

389400 (Human); 404194 (Mouse); 501023 (Rat)

Gene Symbol

GFRAL

UniProt

Q6SJE0-1

Additional GFR alpha-like Products

Product Documents for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

For research use only

Related Research Areas

GDNF Family

Citations for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

Customer Reviews for Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes