Recombinant Mouse Hepsin Protein, CF

R&D Systems | Catalog # 9156-SE

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Hepsin Protein (9156-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O35453-2

Applications

Enzyme Activity
View all Hepsin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Hepsin protein
Gln44-Pro416 (Asp160Glu & Arg161Lys), with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln44 inferred from enzymatic pyroglutamate treatment revealing Ser45

Predicted Molecular Mass

42 kDa

SDS-PAGE

41-47 kDa, reducing conditions

Activity

Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC).
The specific activity is >8,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 9156-SE in the following applications:

Formulation, Preparation, and Storage

9156-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hepsin

Hepsin, also known as TMPRSS1, is an approximately 50 kDa Type II membrane protein with an extracellular serine protease domain. It is primarily expressed in liver as a disulfide linked heterodimer of heavy and light chains (1, 2). Hepsin cleaves and activates Coagulation Factors VII, IX, and XII (3, 4), the pro forms of HGF, MSP, and uPA (4-8), and Prostasin/PRSS8 (9). It also cleaves EGF R and prevents receptor binding of EGF [9]. Its enzymatic activity is inhibited by HAI-1 and HAI-2 (4, 5, 8). Hepsin is overexpressed in various human tumors including prostate and breast (6, 10), and it promotes tumor progression and metastasis (11). It disrupts attachment of epithelial cells to the basement membrane by inducing the breakdown of  desmosomal structures (6). Hepsin also binds to Protein X from hepatitis B virus and to pORF3 from hepatitis E virus (12, 13). Within the ECD, mouse Hepsin shares 89% and 98% amino acid (aa) sequence identity with human and rat Hepsin, respectively (2). An alternatively spliced isoform has a 20 aa deletion in the cytoplasmic and transmembrane regions (2).

References

  1. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  2. Vu, T.-K.H. et al. (1997) J. Biol. Chem. 272:31315.
  3. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  4. Herter, S. et al. (2005) Biochem. J. 390:125.
  5. Kirchhofer, D. et al. (2005) FEBS Lett. 579:1945.
  6. Tervonen, T.A. et al. (2016) Oncogene 35:1832.
  7. Ganesan, R. et al. (2011) Mol. Cancer Res. 9:1175.
  8. Moran, P. et al. (2006) J. Biol. Chem. 281:30439.
  9. Chen, M. et al. (2010) Mol. Cell. Biochem. 337:259.
  10. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  11. Li, W. et al. (2009) Cancer Res. 69:8395.
  12. Zhang, M. et al. (2015) Oncotarget 7:7780.
  13. Wang, C. et al. (2014) Hepat. Mon. 14:e13902.

Alternate Names

HPN, TMPRSS1

Entrez Gene IDs

3249 (Human); 15451 (Mouse); 29135 (Rat)

Gene Symbol

HPN

UniProt

O35453-2

Additional Hepsin Products

Product Documents for Recombinant Mouse Hepsin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Hepsin Protein, CF

For research use only

Customer Reviews for Recombinant Mouse Hepsin Protein, CF (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Mouse Hepsin Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Recombinant Mouse Hepsin Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/04/2017
    - added 50 uL of 50 uM of substrate in each well - added 1, 2,3,4, or 5 ng in each well

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

View specific protocols for Recombinant Mouse Hepsin Protein, CF (9156-SE):

Materials
  •  Activation Buffer: 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 8.0
  • Assay Buffer: 50 mM Tris, pH 9.0
  • Recombinant Mouse Hepsin (rmHepsin) (Catalog # 9156-SE)
  • Substrate: BOC-Gln-Arg-Arg-AMC (Bachem, Catalog # I-1655), 5 mM stock in 50:50 DMSO:Methanol
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmHepsin to 100 µg/mL in Activation Buffer.
  2. Incubate at 37 °C for 24 hours.
  3. Dilute activated rmHepsin to 0.2 µg/mL in Assay Buffer.
  4. Dilute Substrate to 200 μM in Assay Buffer.
  5. Load into a plate 50 μL of 0.2 µg/mL rmHepsin, and start the reaction by adding 50 μL of 200 μM Substrate. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of 200 μM Substrate.
  6. Read plate at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
  7. Calculate specific activity:

 

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank.
     **Derived using calibration standard 7-Amino, 4-Methyl Coumain (AMC) (Sigma, Catalog # A9891).

Per Well:
  • rmHepsin: 0.010 μg
  • Substrate: 100 μM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes