Recombinant Mouse HGF Propeptide Protein, CF

R&D Systems | Catalog # 7058-HG

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Key Product Details

  • R&D Systems Sf 21 (stably transfected)-derived Recombinant Mouse HGF Propeptide Protein (7058-HG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (stably transfected)

Accession Number

Q08048

Applications

Bioactivity
View all HGF Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse HGF protein
Gln33-Leu728

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln33 predicted, sequencing might be blocked

Predicted Molecular Mass

79.3 kDa

SDS-PAGE

84 kDa, reducing conditions

Activity

>10 ng of Recombinant Mouse HGF Propeptide is cleaved to release active HGF when treated with kallikrein.

Formulation, Preparation, and Storage

7058-HG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-7). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates an additional HGF isoform with a 5 aa deletion in the first Kringle domain. Mouse Pro‑HGF shares 92%-93% aa sequence identity with bovine, canine, feline, and human Pro-HGF and 99% with rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (8, 9). HGF-dependent c-MET activation is implicated in the development of many human cancers (10). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (11, 12). HGF induces the up-regulation of Integrin  alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (13). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (13, 14). HGF can also alter epithelium morphology by the induction of Nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (15). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (16). HGF promotes the motility of cardiac stem cells in damaged myocardium (17).

References

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Liu, Y. et al. (1993) Biochim. Biophys. Acta 1216:299.
  7. Lee, C.C. et al. (1993) Cell Adhes. Commun. 1:101.
  8. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  9. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  10. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  11. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  12. Montesano, R. et al. (1991) Cell 67:901.
  13. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  14. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  15. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  16. Mineo, R. et al. (1994) Endocrinology 145:4355.
  17. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Gene Symbol

HGF

UniProt

Q08048

Additional HGF Products

Product Documents for Recombinant Mouse HGF Propeptide Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse HGF Propeptide Protein, CF

For research use only

Citations for Recombinant Mouse HGF Propeptide Protein, CF

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