Recombinant Mouse ICAM-1/CD54 His-tag Protein, CF Summary
The ED50 for this effect is 0.075-0.6 µg/mL.
Gln28-Asn485, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse ICAM-1/CD54 His-tag Protein (Catalog # 10304-IC) supports the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.075-0.6 μg/mL.
2 μg/lane of Recombinant Mouse ICAM-1/CD54 His-tag (Catalog # 10304-IC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa.
ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane cell adhesion glycoprotein and a member of the immunoglobulin supergene family. The ICAM sub-family consists of five members, ICAM-1 through ICAM-5, and they vary in their tissue expression and number of Ig-like domains in the extracellular domain (ECD). Full-length ICAM-1 contains 5 Ig-like domains in the ECD, a single transmembrane domain and short intracellular domain. Alternative splicing of ICAM-1 results in at least six membrane-bound forms and one soluble form (1). The ECD of mature, full-length mouse ICAM-1 shares 78% and 54% amino acid sequence identity with rat and human ICAM-1, respectively. ICAM-1 plays an important role in both innate and adaptive immune responses and is up-regulated on endothelial and epithelial cells at sites of inflammation (2, 3). ICAM-1 mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) (2, 3). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes (4-8). Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage (9). Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria (10-18). More recently, ICAM-1 was shown to be a novel regulator of group 2 innate lymphoid cells (ILC2s) which play a key role in allergic airway inflammation (19).
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